INHIBITION OF AGS CANCER CELL PROLIFERATION FOLLOWING SIRNA-MEDIATED DOWNREGULATION OF VEGFR2

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ZAREI MAHMOUDABADI ALI; MASOOMI KARIMI MASOOMEH; BAHABADI MAJID; BAGHERI HOSSEIN ABADI ZAHRA; JAFARISANI MOSLEM; AHMADI REZA

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Journal: Cell Journal (Yakhteh) Year:2016 | Volume:18 | Issue:3 (71) Page(s): 381-388

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Title

INHIBITION OF AGS CANCER CELL PROLIFERATION FOLLOWING SIRNA-MEDIATED DOWNREGULATION OF VEGFR2

Author(s)

Keywords

VEGFR2

DOWNREGULATION

SIRNA

APOPTOSIS

Abstract

Objective: Vascular endothelial growth factor (VEGF) and VEGF receptors (VEGFRs) play important roles in angiogenesis of different developmental mechanisms such as wound healing, embryogenesis and diseases, including different types of cancer. VEGFR2 is associated with cell proliferation, migration, and vascular permeability of endothelial cells.Blocking VEGF and its receptors is suggested as a therapeutic approach to prevent tumor growth. In this study, we aim to block VEGF signaling via small interfering RNA (siRNA) inhibition of VEGFR2.Materials and Methods: In this experimental study, we used the RNA interference (RNAi) mechanism to suppress expression of theVEGFR2 gene. We conducted the 3- (4, 5-dimethylthiazol-2-yl) -2, 5-diphenyl-tetrazolium bromide (MTT) assay, real-time polymerase chain reaction (PCR), Western blot, and flow cytometry analyses of VEGFR2 expression.Results: Real-time PCR and Western blot results showed that VEGFR2 expression significantly downregulated. This suppression was followed by inhibition of cell proliferation, reduction of viability, and induction of APOPTOSIS in the cancer cells.Conclusion: These findings suggest that VEGFR2 has a role in cell proliferation and tumor growth. Accordingly, it is suggested that VEGFR2 can be a therapeutic target for controlling tumor growth and proliferation.

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APA: Copy

ZAREI MAHMOUDABADI, ALI, MASOOMI KARIMI, MASOOMEH, BAHABADI, MAJID, BAGHERI HOSSEIN ABADI, ZAHRA, JAFARISANI, MOSLEM, & AHMADI, REZA. (2016). INHIBITION OF AGS CANCER CELL PROLIFERATION FOLLOWING SIRNA-MEDIATED DOWNREGULATION OF VEGFR2. CELL JOURNAL (YAKHTEH), 18(3 (71)), 381-388. SID. https://sid.ir/paper/683930/en

Vancouver: Copy

ZAREI MAHMOUDABADI ALI, MASOOMI KARIMI MASOOMEH, BAHABADI MAJID, BAGHERI HOSSEIN ABADI ZAHRA, JAFARISANI MOSLEM, AHMADI REZA. INHIBITION OF AGS CANCER CELL PROLIFERATION FOLLOWING SIRNA-MEDIATED DOWNREGULATION OF VEGFR2. CELL JOURNAL (YAKHTEH)[Internet]. 2016;18(3 (71)):381-388. Available from: https://sid.ir/paper/683930/en

IEEE: Copy

ALI ZAREI MAHMOUDABADI, MASOOMEH MASOOMI KARIMI, MAJID BAHABADI, ZAHRA BAGHERI HOSSEIN ABADI, MOSLEM JAFARISANI, and REZA AHMADI, “INHIBITION OF AGS CANCER CELL PROLIFERATION FOLLOWING SIRNA-MEDIATED DOWNREGULATION OF VEGFR2,” CELL JOURNAL (YAKHTEH), vol. 18, no. 3 (71), pp. 381–388, 2016, [Online]. Available: https://sid.ir/paper/683930/en

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