Cytotoxic effects of hydroalcoholic extract of Cuscuta chinensis on PC3 and MCF7 cancer cell lines

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Karimi Dermani Fatemeh; SAIDIJAM MASSOUD; NAJAFI REZVAN; MORADKHANI SHIRIN; Mohammadzaheri Zahra; Beiranvand Negar; MOHAMMADI SAMANEH; Shabab Noushin; KALVANDI RAMAZAN; ZERAATI FATEMEH

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Journal: Avicenna Journal of Phytomedicine Year:2021 | Volume:11 | Issue:3 Page(s): 258-268

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Title

Cytotoxic effects of hydroalcoholic extract of Cuscuta chinensis on PC3 and MCF7 cancer cell lines

Author(s)

Keywords

Cuscuta

chinensis

Chemoprevention

Prostate cancer

Breast cancer

Apoptosis

Abstract

Objective: Chemoprevention of cancer by application of natural phytochemical compounds has been used to prevent, delay or suppress cancer progression. Cuscuta chinensis a traditional Iranian medicinal herb, has biological properties including anticancer, antiaging, immuno-stimulatory and antioxidant effects. In this study, anti-proliferative effects of hydroalcoholic extract of C. chinensis on prostate (PC3) and breast (MCF7) cancer cell lines were investigated. Materials and Methods: In the current study, we investigated treatment of PC3 cells with different concentrations of C. chinensis (0, 100, 200, 300, 400, and 500 μ g/ml) for 24 and 48 hr; also, MCF7 cells were treated with various concentrations (0-600 μ g/ml) of C. chinensis for 48 and 72 hr and cell viability was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. mRNA expression of BCL2 Associated X (Bax), B-cell lymphoma 2 (Bcl2), Cysteine-aspartic proteases (Caspase3) and Phosphatase and tensin homolog (PTEN) were analyzed by quantitative real-time PCR. Annexin V/PI staining and lactate dehydrogenase (LDH) cytotoxicity assay were used to detect Apoptosis. Results: C. chinensis decreased PC3 and MCF7 cells viability in a dose-and time-dependent manner (p<0. 01 to p<0. 001). The gene expression of BAX/Bcl2 ratio, Caspase3 and PTEN increased in C. chinensis-treated cells compared to the control group. C. chinensis induced Apoptosis (p<0. 001) and LDH activity (p<0. 01 to p<0. 001). Conclusion: Our findings suggest that C. chinensis extract is able to inhibit proliferation and induce Apoptosis in PC3 and MCF7 cell lines. Therefore, C. chinensis extract exerts antitumor activity against cancer cells.

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APA: Copy

Karimi Dermani, Fatemeh, SAIDIJAM, MASSOUD, NAJAFI, REZVAN, MORADKHANI, SHIRIN, Mohammadzaheri, Zahra, Beiranvand, Negar, MOHAMMADI, SAMANEH, Shabab, Noushin, KALVANDI, RAMAZAN, & ZERAATI, FATEMEH. (2021). Cytotoxic effects of hydroalcoholic extract of Cuscuta chinensis on PC3 and MCF7 cancer cell lines. AVICENNA JOURNAL OF PHYTOMEDICINE (AJP), 11(3), 258-268. SID. https://sid.ir/paper/684074/en

Vancouver: Copy

Karimi Dermani Fatemeh, SAIDIJAM MASSOUD, NAJAFI REZVAN, MORADKHANI SHIRIN, Mohammadzaheri Zahra, Beiranvand Negar, MOHAMMADI SAMANEH, Shabab Noushin, KALVANDI RAMAZAN, ZERAATI FATEMEH. Cytotoxic effects of hydroalcoholic extract of Cuscuta chinensis on PC3 and MCF7 cancer cell lines. AVICENNA JOURNAL OF PHYTOMEDICINE (AJP)[Internet]. 2021;11(3):258-268. Available from: https://sid.ir/paper/684074/en

IEEE: Copy

Fatemeh Karimi Dermani, MASSOUD SAIDIJAM, REZVAN NAJAFI, SHIRIN MORADKHANI, Zahra Mohammadzaheri, Negar Beiranvand, SAMANEH MOHAMMADI, Noushin Shabab, RAMAZAN KALVANDI, and FATEMEH ZERAATI, “Cytotoxic effects of hydroalcoholic extract of Cuscuta chinensis on PC3 and MCF7 cancer cell lines,” AVICENNA JOURNAL OF PHYTOMEDICINE (AJP), vol. 11, no. 3, pp. 258–268, 2021, [Online]. Available: https://sid.ir/paper/684074/en

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