PROTECTIVE EFFECTS OF FRACTIONS FROM ARTEMISIA BIENNIS HYDRO-ETHANOLIC EXTRACT AGAINST DOXORUBICIN-INDUCED OXIDATIVE STRESS AND APOPTOSIS IN PC12 CELLS

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MOJARRAB MAHDI; MEHRABI MEHRAN; AHMADI FARAHNAZ; HOSSEINZADEH LEILA

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Journal: Iranian Journal of Basic Medical Sciences Year:2016 | Volume:19 | Issue:5 Page(s): 503-510

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Title

PROTECTIVE EFFECTS OF FRACTIONS FROM ARTEMISIA BIENNIS HYDRO-ETHANOLIC EXTRACT AGAINST DOXORUBICIN-INDUCED OXIDATIVE STRESS AND APOPTOSIS IN PC12 CELLS

Author(s)

MOJARRAB MAHDI | MEHRABI MEHRAN | AHMADI FARAHNAZ | HOSSEINZADEH LEILA | Issue Writer Certificate

Keywords

APOPTOSIS

ARTEMISIA BIENNIS

DOXORUBICIN

OXIDATIVE STRESS

PC12 CELL LINE

Abstract

Objective (s): This study was designed to indicate whether different fractions from ARTEMISIA BIENNIS hydroethanolic extract could provide cytoprotection against OXIDATIVE STRESS and APOPTOSIS induced by DOXORUBICIN (DOX) in rat pheochromocytoma cell line (PC12).Material and Methods: Cell viability was determined by MTT assay. Also, activation of caspase-3 and superoxide dismutase were evaluated by spectrophotometry. Detection of reactive oxygen species (ROS) and measurement of mitochondrial membrane potential (MMP) were performed by flowcytometry.Results: Treatment of PC12 cells with DOX reduced viability dose dependently. For evaluation of the effect of fractions (A-G) on DOX-induced cytotoxicity, PC12 cells were pretreated for 24 hr with the A. biennis fractions and then cells were treated with DOX. The fractions C and D increased PC12 cells viability significantly compared to DOX treated cells. Moreover, pretreatment with fractions C and D for 24 hr attenuated DOX-mediated APOPTOSIS and the anti-apoptotic action of A. biennis fractions was partially dependent on inhibition of caspase 3 activity and also increasing the mitochondrial membrane potential (MMP). Selected A. biennis fractions also suppressed the generation of ROS and increased superoxide dismutase (SOD) activity.Conclusion: Taken together our observation indicated that subtoxic concentration of aforementioned fractions of A. biennis hydroetanolic extract has protective effect against APOPTOSIS induced by DOX in PC12 cell. The results highlighted that fractions C and D may exert cytoprotective effects through their antioxidant actions.

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APA: Copy

MOJARRAB, MAHDI, MEHRABI, MEHRAN, AHMADI, FARAHNAZ, & HOSSEINZADEH, LEILA. (2016). PROTECTIVE EFFECTS OF FRACTIONS FROM ARTEMISIA BIENNIS HYDRO-ETHANOLIC EXTRACT AGAINST DOXORUBICIN-INDUCED OXIDATIVE STRESS AND APOPTOSIS IN PC12 CELLS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 19(5), 503-510. SID. https://sid.ir/paper/689472/en

Vancouver: Copy

MOJARRAB MAHDI, MEHRABI MEHRAN, AHMADI FARAHNAZ, HOSSEINZADEH LEILA. PROTECTIVE EFFECTS OF FRACTIONS FROM ARTEMISIA BIENNIS HYDRO-ETHANOLIC EXTRACT AGAINST DOXORUBICIN-INDUCED OXIDATIVE STRESS AND APOPTOSIS IN PC12 CELLS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2016;19(5):503-510. Available from: https://sid.ir/paper/689472/en

IEEE: Copy

MAHDI MOJARRAB, MEHRAN MEHRABI, FARAHNAZ AHMADI, and LEILA HOSSEINZADEH, “PROTECTIVE EFFECTS OF FRACTIONS FROM ARTEMISIA BIENNIS HYDRO-ETHANOLIC EXTRACT AGAINST DOXORUBICIN-INDUCED OXIDATIVE STRESS AND APOPTOSIS IN PC12 CELLS,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 19, no. 5, pp. 503–510, 2016, [Online]. Available: https://sid.ir/paper/689472/en

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