LACTOBACILLI MODULATE HYPOXIA-INDUCIBLE FACTOR (HIF) -1 REGULATORY PATHWAY IN TRIPLE NEGATIVE BREAST CANCER CELL LINE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ESFANDIARI ALI; TAHERIAN ESFAHANI ZAHRA; ABEDIN DO ATIEH; MIRFAKHRAEI REZA; SHIRZAD MAHDIEH; GHAFOURI FARD SOUDEH; MOTEVASELI ELAHE

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2016 | Volume:18 | Issue:2 (70) Page(s): 237-244

Download Full-Text

View:

497

Download:

235

Cites:

Information Journal Paper

Title

LACTOBACILLI MODULATE HYPOXIA-INDUCIBLE FACTOR (HIF) -1 REGULATORY PATHWAY IN TRIPLE NEGATIVE BREAST CANCER CELL LINE

Author(s)

Keywords

BREAST CANCER

LACTOBACILLUS

HIF

Abstract

Objective: Hypoxia-Inducible Factor (HIF) -1 plays an essential role in the body’s response to low oxygen concentrations and regulates expression of several genes implicated in homeostasis, vascularization, anaerobic metabolism as well as immunological responses. Increased levels of HIF-1a are associated with increased proliferation and more aggressive breast tumor development. Lactobacilli have been shown to exert anti-cancer effects on several malignancies including BREAST CANCER. However, the exact mechanism of such effect is not clear yet.The aim of this study was to analyze the expression of selected genes from HIF pathway in a triple negative BREAST CANCER cell line (expressing no estrogen and progesterone receptors as well as HER-2/Neu), MDA-MB-231, following treatment with two lactobacilli culture supernatants.Materials and Methods: In this experimental study, we analyzed the expression of HIF-1a, SLC2A1, VHL, HSP90, XBP1and SHARP1 genes from HIF pathway in MDA-MB-231 cells, before and after treatment withLactobacillus crispatus and LACTOBACILLUS rhamnosus culture supernatants (LCS and LRS, respectively) by means of quantitative reverse-transcription polymerase chain reaction (qRT-PCR).Results: Both LRS and LCS had cytotoxic effects on MDA-MB-231 cells, while the former type was more cytotoxic. LRS dramatically down-regulated expression levels of theHIF-1a, HSP90and SLC2A1 in the MDA-MB-231 cells. LCS had similar effect on the expression ofHSP90, to what was observed in the LRS treatment. The expression level of tumor suppressor genesVHL and SHARP1 were also decreased in LCS treated cells.Conclusion: Although both LCS and LRS had cytotoxic effects on the MDA-MB-231 cells, it is proposed that LRS could be more appropriate for pathway directed treatment modalities, as it did not decrease expression of tumor suppressor genes involved in HIF pathway.Down-regulation of HIF pathway mediated oncogenes by LRS suggests that the cytotoxic effects of thislactobacillus may at least be partly caused by this mechanism. As previous studies have shown that inhibition of HIF-1a and HSP90 expressions have therapeutic impact on cancer treatment, the inhibitory effect of LRS on expression of these genes implies that thislactobacillus can be used in treatment strategies.

Cites

No record.

References

No record.

Cite

APA: Copy

ESFANDIARI, ALI, TAHERIAN ESFAHANI, ZAHRA, ABEDIN DO, ATIEH, MIRFAKHRAEI, REZA, SHIRZAD, MAHDIEH, GHAFOURI FARD, SOUDEH, & MOTEVASELI, ELAHE. (2016). LACTOBACILLI MODULATE HYPOXIA-INDUCIBLE FACTOR (HIF) -1 REGULATORY PATHWAY IN TRIPLE NEGATIVE BREAST CANCER CELL LINE. CELL JOURNAL (YAKHTEH), 18(2 (70)), 237-244. SID. https://sid.ir/paper/689482/en

Vancouver: Copy

ESFANDIARI ALI, TAHERIAN ESFAHANI ZAHRA, ABEDIN DO ATIEH, MIRFAKHRAEI REZA, SHIRZAD MAHDIEH, GHAFOURI FARD SOUDEH, MOTEVASELI ELAHE. LACTOBACILLI MODULATE HYPOXIA-INDUCIBLE FACTOR (HIF) -1 REGULATORY PATHWAY IN TRIPLE NEGATIVE BREAST CANCER CELL LINE. CELL JOURNAL (YAKHTEH)[Internet]. 2016;18(2 (70)):237-244. Available from: https://sid.ir/paper/689482/en

IEEE: Copy

ALI ESFANDIARI, ZAHRA TAHERIAN ESFAHANI, ATIEH ABEDIN DO, REZA MIRFAKHRAEI, MAHDIEH SHIRZAD, SOUDEH GHAFOURI FARD, and ELAHE MOTEVASELI, “LACTOBACILLI MODULATE HYPOXIA-INDUCIBLE FACTOR (HIF) -1 REGULATORY PATHWAY IN TRIPLE NEGATIVE BREAST CANCER CELL LINE,” CELL JOURNAL (YAKHTEH), vol. 18, no. 2 (70), pp. 237–244, 2016, [Online]. Available: https://sid.ir/paper/689482/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops