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Information Journal Paper

Title

ULTRASOUND EFFECT ON GENE EXPRESSION OF SEX DETERMINING REGION Y-BOX 9 (SOX9) AND TRANSFORMING GROWTH FACTOR β ISOFORMS IN ADIPOSE STEM CELLS

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Abstract

 Background: CARTILAGE TISSUE ENGINEERING is a promising method for repair of cartilage defects. Induction of CHONDROGENESIS in mesenchymal stem cells (MSC) is currently used in CARTILAGE TISSUE ENGINEERING. Among growth factors, transforming growth factor b (TGF-b) iscommonchondrogenic inducer but toward hypertrophic chondrocyte. However, mechanical factors such as ULTRASOUND could stimulate CHONDROGENESIS.Objectives: We aimed to investigate stimulation of endogenous TGF-b genes expression by low intensity pulsed ULTRASOUND (LIPUS) in MSC.Materials and Methods: In this experimental study, adipose tissue stem cells (ASC) cultures were treated with or without LIPUS (30 mW/cm2, 20 min/day) and with or without TGF-b3 (10 ng/mL) for 4 or 14 days. Chondrogenic gene expression of SOX9 and members of TGF-b family (b1, b2 and b3) was assessed in ASC cultures at day 4 and 14 by real time PCR.Results: The gene expression of SOX9 significantly increased by LIPUS and TGF-b treatment versus control cultures. Exogenous TGF-b3 treatment stimulated endogenous TGF-b1 and b2 gene expressions more than LIPUS treated cultures at day 4. LIPUS, TGF-b and LIPUS plus TGF-b treated cultures expressed same TGF-b3 gene expression at day 4. The expression of TGF-b1 and b2 decreased by LIPUS in comparison to TGF-b treated cultures at day 14.Conclusions: Our results suggest that LIPUS might initiate differentiation of ASC without enhancing endogenous TGF-b genes in in-vitro.

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    APA: Copy

    SHAFAEI, HAJAR, & BAGHERNEZHAD, HASSAN. (2016). ULTRASOUND EFFECT ON GENE EXPRESSION OF SEX DETERMINING REGION Y-BOX 9 (SOX9) AND TRANSFORMING GROWTH FACTOR β ISOFORMS IN ADIPOSE STEM CELLS. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), 18(4), 0-0. SID. https://sid.ir/paper/689484/en

    Vancouver: Copy

    SHAFAEI HAJAR, BAGHERNEZHAD HASSAN. ULTRASOUND EFFECT ON GENE EXPRESSION OF SEX DETERMINING REGION Y-BOX 9 (SOX9) AND TRANSFORMING GROWTH FACTOR β ISOFORMS IN ADIPOSE STEM CELLS. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH)[Internet]. 2016;18(4):0-0. Available from: https://sid.ir/paper/689484/en

    IEEE: Copy

    HAJAR SHAFAEI, and HASSAN BAGHERNEZHAD, “ULTRASOUND EFFECT ON GENE EXPRESSION OF SEX DETERMINING REGION Y-BOX 9 (SOX9) AND TRANSFORMING GROWTH FACTOR β ISOFORMS IN ADIPOSE STEM CELLS,” ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), vol. 18, no. 4, pp. 0–0, 2016, [Online]. Available: https://sid.ir/paper/689484/en

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