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Title

THE IDENTIFICATION OF CHEMICAL AND BACTERIAL COMPOSITION AND DETERMINATION OF FIMH GENE FREQUENCY OF KIDNEY STONES OF IRANIAN PATIENTS

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Abstract

 Background: There is rare study on the association between FimH and KIDNEY STONE formation in our country.Objectives: Here we studied on stones and identified the bacteria in stones isolated from KIDNEY STONE disease and/or UTI patients attending to Hashemi-Nejad hospital (Tehran, IRAN) to find out a possible correlation between stone composition and the diseases. We also measure the frequency of fimH gene and its related protein in Escherichia coli isolated from the patients to clarify the effect of this gene in KIDNEY STONE formation.Patients and Methods: In This observational-descriptive study, 40 KIDNEY STONE samples were gathered and the composition of each sample was determined. The frequency of fimH gene and its related protein was measured using PCR and protein extraction from separated E. coli bacteria.Results: The most prevalence of stones belonged to calcium oxalate stones and the most frequent bacterium in KIDNEY STONEs was E. coli. The frequency of fimH gene in isolated E. coli was 57.14%.Conclusions: Our data indicated that almost all chemical types of KIDNEY STONEs may involve in UTI and KIDNEY STONE formation. We also realized that although E. coli is a non-urea splitting bacteria, it is the most causative microorganism found in urine and stones. Finally we recognized that fimH gene is seen in the majority of KIDNEY STONE samples so it may have a role in formation of KIDNEY STONE, although it should be more clarified in future studies.

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    APA: Copy

    SHOJAIYAN, ALI, ROSTAMIAN, MOSAYEB, NOROUZI, JAMILEH, & PAKZAD, PARVIZ. (2016). THE IDENTIFICATION OF CHEMICAL AND BACTERIAL COMPOSITION AND DETERMINATION OF FIMH GENE FREQUENCY OF KIDNEY STONES OF IRANIAN PATIENTS. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), 18(6), 0-0. SID. https://sid.ir/paper/689485/en

    Vancouver: Copy

    SHOJAIYAN ALI, ROSTAMIAN MOSAYEB, NOROUZI JAMILEH, PAKZAD PARVIZ. THE IDENTIFICATION OF CHEMICAL AND BACTERIAL COMPOSITION AND DETERMINATION OF FIMH GENE FREQUENCY OF KIDNEY STONES OF IRANIAN PATIENTS. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH)[Internet]. 2016;18(6):0-0. Available from: https://sid.ir/paper/689485/en

    IEEE: Copy

    ALI SHOJAIYAN, MOSAYEB ROSTAMIAN, JAMILEH NOROUZI, and PARVIZ PAKZAD, “THE IDENTIFICATION OF CHEMICAL AND BACTERIAL COMPOSITION AND DETERMINATION OF FIMH GENE FREQUENCY OF KIDNEY STONES OF IRANIAN PATIENTS,” ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), vol. 18, no. 6, pp. 0–0, 2016, [Online]. Available: https://sid.ir/paper/689485/en

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