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Information Journal Paper

Title

Enrichment and Rapid Detection of Vibrio Cholerae From Water by Non-culture Method

Pages

  902-904

Abstract

 Background and Aim In the microbial contamination of food and water, identifying the trace amounts of contaminating bacteria has always been of researchers’ interest and concern. The most frequent approach to resolve this problem is using culture-based methods to increase and enrich bacteria samples; accordingly, it extends the bacterial Detection process to several hours or days. One of the smart strategies to solve this problem is the concentration of bacteria using physical methods. The present study aimed to enrich Vibrio cholerae as the most essential water-polluting germs. Accordingly, we used the Filtration method and evaluated its function by culture method and two Detection approaches of Adenosine Triphosphate (ATP) and PCR assay. Methods & Materials A certain concentration of V. Cholerae was artificially added to a specified volume of sterile water. Then, the bacteria were extracted from the medium and filtered using 0. 450 μ m separable filters. Finally, the performance of the pre-and post-Filtration processes was compared using bacterial cell culture (CFU), ATP, and PCR assay with the specific primers for the ompW gene of V. cholerae. Ethical Considerations This article is a meta-analysis with no human or animal sample. Results The present research results indicated that the applied method presented high efficiency and recovery performance. In other words, samples provided no positive response before Filtration in both methods; however, after Filtration in isolated and recovered samples, the presence of bacteria was detected in the ATP and PCR methods. Conclusion In conclusion, the employed strategy can detect V. cholerae in non-culture and in the shortest time in contaminated water samples.

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    • Cite

    APA: Copy

    MORADI, ABOLFAZL, & ZEINODDINI, MEHDI. (2021). Enrichment and Rapid Detection of Vibrio Cholerae From Water by Non-culture Method. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 23(6 (148) ), 902-904. SID. https://sid.ir/paper/691175/en

    Vancouver: Copy

    MORADI ABOLFAZL, ZEINODDINI MEHDI. Enrichment and Rapid Detection of Vibrio Cholerae From Water by Non-culture Method. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2021;23(6 (148) ):902-904. Available from: https://sid.ir/paper/691175/en

    IEEE: Copy

    ABOLFAZL MORADI, and MEHDI ZEINODDINI, “Enrichment and Rapid Detection of Vibrio Cholerae From Water by Non-culture Method,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 23, no. 6 (148) , pp. 902–904, 2021, [Online]. Available: https://sid.ir/paper/691175/en

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