Cloning, Expression, and Purification of Recombinant Mouse Interferon-γ

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Badiee Kheirabadi Seyedeh Elham; Mashayekhi Kazem; Moghadam Malihe; MOUSAVI MOHAMMAD JAVAD; SANKIAN MOJTABA

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Journal: Research in Molecular Medicine Year:2021 | Volume:9 | Issue:1 Page(s): 1-9

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Title

Cloning, Expression, and Purification of Recombinant Mouse Interferon-γ

Author(s)

Keywords

Cloning

E.coli BL21 (DE3) CodonPlus

pET-21b(+) vector

Interferon-γ

Recombinant protein

Abstract

Background: Interferon-gamma (IFN-γ ) is the most important cytokine in the immune system. This protein has been expressed in bacterial cells. However, bacterial Cloning is not an easy task. We aimed to clone, express, and purify recombinant mouse IFN-γ and overcome problems in favor of commercial purposes. Materials and Methods: To amplify the gene product for Cloning, we primarily designed two specific primers for the target gene. Following PCR amplification, the amplicon was inserted into the pET-21b(+) vector. The E. coli BL21 (DE3) CodonPlus strain was chosen for the expression of the target gene. Finally, the expressed recombinant mouse IFN-γ was assessed through the western blotting method. Results: We performed a Cloning process and produced recombinant mouse IFN-γ in an optimal condition. We also noticed that monomeric protein could be transformed to a homodimeric structure which can be observed using the SDS PAGE (SDS-polyacrylamide gel electrophoresis) and western blotting. Conclusion: Experimental conditions strongly affect the large-scale Cloning procedures required to be optimized in each laboratory. The expressed recombinant mouse IFN-γ described here is appropriate for commercial purposes.

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APA: Copy

Badiee Kheirabadi, Seyedeh Elham, Mashayekhi, Kazem, Moghadam, Malihe, MOUSAVI, MOHAMMAD JAVAD, & SANKIAN, MOJTABA. (2021). Cloning, Expression, and Purification of Recombinant Mouse Interferon-γ. RESEARCH IN MOLECULAR MEDICINE, 9(1), 1-9. SID. https://sid.ir/paper/691451/en

Vancouver: Copy

Badiee Kheirabadi Seyedeh Elham, Mashayekhi Kazem, Moghadam Malihe, MOUSAVI MOHAMMAD JAVAD, SANKIAN MOJTABA. Cloning, Expression, and Purification of Recombinant Mouse Interferon-γ. RESEARCH IN MOLECULAR MEDICINE[Internet]. 2021;9(1):1-9. Available from: https://sid.ir/paper/691451/en

IEEE: Copy

Seyedeh Elham Badiee Kheirabadi, Kazem Mashayekhi, Malihe Moghadam, MOHAMMAD JAVAD MOUSAVI, and MOJTABA SANKIAN, “Cloning, Expression, and Purification of Recombinant Mouse Interferon-γ,” RESEARCH IN MOLECULAR MEDICINE, vol. 9, no. 1, pp. 1–9, 2021, [Online]. Available: https://sid.ir/paper/691451/en

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