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Information Journal Paper

Title

EXPRESSION OPTIMIZING AND PURIFICATION OF RECOMBINANT HUMAN LEUKEMIA INHIBITORY FACTOR PRODUCED IN E. COLI STRAIN BL21

Pages

  0-0

Abstract

 Background: Leukemia INHIBITORY FACTOR (LIF) is a glycoprotein, categorized as asubfamily of interleukin 6 cytokines which is known in many mammolals. A pluripotentcytokine with a wide biological function range has numerous effects on target cells. TheLIF regulates neuron survival, hematopoiesis and seen in LIF-/- knockout mice affectsblastocyst implantation, also acts as pre-inflammolatory cytokine, and regulates immoluneresponse. Further, it is able to maintain stem cells poly potency. The main object ofpresent work was expression, optimizing, and PURIFICATION of RECOMBINANT HUMAN LEUKEMIAinhibitory factor (rhLIF).Materials and Methods: In this experimental study, Pet28 (+) carrying the LIF gene andkanamycin resistance marker was cloned in E. COLI strain BL21. The induction wasoptimized by altering 3 factors including the temperature, the induction time, and theconcentration of the Isopropyl b-D-1-thiogalactopyranoside (IPTG) as inducer. Thepurification of the recombinant human LIF (rhLIF) was done by single step affinitychromatography. After the PURIFICATION, method accuracy was proved by Sodium dodecylsulfate (SDS) -PAGE electrophoresis and Western blotting.Results: Optimizing of the expression was reached by changing various parameters, andpurification has been done successful.Conclusion: rhLIF undergoes modification by glycosylation to get its full functionality.The produced rhLIF in prokaryotic host in this work is lacking of glycosylation. However, its proper function should be evaluated in further studies.

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    APA: Copy

    kahroba, Houman. (2015). EXPRESSION OPTIMIZING AND PURIFICATION OF RECOMBINANT HUMAN LEUKEMIA INHIBITORY FACTOR PRODUCED IN E. COLI STRAIN BL21. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), 17(2), 0-0. SID. https://sid.ir/paper/692544/en

    Vancouver: Copy

    kahroba Houman. EXPRESSION OPTIMIZING AND PURIFICATION OF RECOMBINANT HUMAN LEUKEMIA INHIBITORY FACTOR PRODUCED IN E. COLI STRAIN BL21. ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH)[Internet]. 2015;17(2):0-0. Available from: https://sid.ir/paper/692544/en

    IEEE: Copy

    Houman kahroba, “EXPRESSION OPTIMIZING AND PURIFICATION OF RECOMBINANT HUMAN LEUKEMIA INHIBITORY FACTOR PRODUCED IN E. COLI STRAIN BL21,” ZAHEDAN JOURNAL OF RESEARCH IN MEDICAL SCIENCES (TABIB-E-SHARGH), vol. 17, no. 2, pp. 0–0, 2015, [Online]. Available: https://sid.ir/paper/692544/en

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