IN VITRO EFFECTS OF ERYTHROPOIETIN STIMULATED HEMATOPOIETIC PROGENITOR CELLS ON OSTEOBLASTIC DIFFERENTIATION

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOSLEHABADI FARAHANI M.; AMIRIZADEH N.; ODI A.; KHORSHIDFAR M.; NIKOUGOFTAR ZARIF M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Arak University Medical Sciences Year:2014 | Volume:17 | Issue:7 (88) Page(s): 88-98

Download Full-Text

Persian Verion

View:

1,098

Download:

Cites:

Information Journal Paper

Title

IN VITRO EFFECTS OF ERYTHROPOIETIN STIMULATED HEMATOPOIETIC PROGENITOR CELLS ON OSTEOBLASTIC DIFFERENTIATION

Author(s)

Keywords

ERYTHROPOIETINQ2

OSTEOBLASTIC DIFFERENTIATIONQ2

CORD BLOODQ2

HEMATOPOIETIC STEM CELLSQ2

MESENCHYMAL STEM CELLSQ2

OSTEOPONTINQ2

OSTEOCALCINQ1

Abstract

Background: Hematopoietic stem cell transplants are routinely used to treat patients with cancers and other disorders of blood and immune systems. Osteoblasts constitute part of the stromal cell support system in marrow for hematopoiesis by participating in the formation of the HSC niche. It is believed that interaction between hematopoietic cells and bone forming osteoblasts regulate each other’s function. It is established that acute blood loss in animal models activates bone formation and niche development because of EPO stimulation. In this experimental study we have examined the coculture of HSCs derived from CORD BLOOD which treated with EPO in vitro, on OSTEOBLASTIC DIFFERENTIATION of MESENCHYMAL STEM CELLS.Materials and Methods: In this experimental study MSCs isolated from bone marrow and cocultured with CD 34+CD38- HSCs isolated from CORD BLOOD. These co-cultured cells were treated with different doses of ERYTHROPOIETIN for 14 days, after that RNA were extracted from MSCs and analysed with RT-PCR to evaluate the expression of OSTEOPONTIN and OSTEOCALCIN. Alizarin red and alkaline phosphatase staining were done for OSTEOBLASTIC DIFFERENTIATION.Results: OSTEOPONTIN and OSTEOCALCIN were expressed in MSCs. Cellular staining were positive for OSTEOBLASTIC DIFFERENTIATION. Differentiated cells expressed osteoblastic markers.Conclusion: These data suggest that EPO regulates the OSTEOBLASTIC DIFFERENTIATION from bone marrow MSCs in vitro.

Cites

No record.

References

Cite

APA: Copy

MOSLEHABADI FARAHANI, M., AMIRIZADEH, N., ODI, A., KHORSHIDFAR, M., & NIKOUGOFTAR ZARIF, M.. (2014). IN VITRO EFFECTS OF ERYTHROPOIETIN STIMULATED HEMATOPOIETIC PROGENITOR CELLS ON OSTEOBLASTIC DIFFERENTIATION. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 17(7 (88)), 88-98. SID. https://sid.ir/paper/69340/en

Vancouver: Copy

MOSLEHABADI FARAHANI M., AMIRIZADEH N., ODI A., KHORSHIDFAR M., NIKOUGOFTAR ZARIF M.. IN VITRO EFFECTS OF ERYTHROPOIETIN STIMULATED HEMATOPOIETIC PROGENITOR CELLS ON OSTEOBLASTIC DIFFERENTIATION. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2014;17(7 (88)):88-98. Available from: https://sid.ir/paper/69340/en

IEEE: Copy

M. MOSLEHABADI FARAHANI, N. AMIRIZADEH, A. ODI, M. KHORSHIDFAR, and M. NIKOUGOFTAR ZARIF, “IN VITRO EFFECTS OF ERYTHROPOIETIN STIMULATED HEMATOPOIETIC PROGENITOR CELLS ON OSTEOBLASTIC DIFFERENTIATION,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 17, no. 7 (88), pp. 88–98, 2014, [Online]. Available: https://sid.ir/paper/69340/en

Related Journal Papers