THE EFFECT OF SHORT TIME EXPOSURE T TO 4°C TEMPERATURE ON THE EXPRESSION PROFILES OF MONO-CARBOXYLIC TRANSPORTER GENES 1, 2, 3, AND 4 IN 4-CELL MOUSE EMBRYOS

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RAMEZANI MINA; HOSSEINI AHMAD; KAZEMI BAHRAM; JANAN A.

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Journal: ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ) Year:2010 | Volume:13 | Issue:2 (51) Page(s): 55-62

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Title

THE EFFECT OF SHORT TIME EXPOSURE T TO 4°C TEMPERATURE ON THE EXPRESSION PROFILES OF MONO-CARBOXYLIC TRANSPORTER GENES 1, 2, 3, AND 4 IN 4-CELL MOUSE EMBRYOS

Author(s)

RAMEZANI MINA | HOSSEINI AHMAD | KAZEMI BAHRAM | JANAN A. | Issue Writer Certificate

Keywords

FOUR-CELL EMBRYOQ3

MICEQ2

MONO-CARBOXYLIC TRANSPORTER GENESQ3

PCRQ2

Abstract

Background: One way of embryo preservation is cryopreservation, but this process may damage and lead to the loss of the embryos, and bring about chromosomal abnormality. This has led researchers to seek techniques for short term preservation of embryos in 0-10 oC temperatures. The aim of this study was to determine the effect of short time exposure to 4oC temperature on the expression profiles of MONO-CARBOXYLIC TRANSPORTER GENES 1, 2, 3, and 4(MCT1-4) in 4-cell mouse embryos.Materials and Methods: In this fundamental study, forty 4-cell mouse embryos from NMRI strain were randomly divided into two groups. The first group consisted of fresh 4-cell embryos, and the second group included 4-cell mouse embryos that were exposed to 4oC temperature for 24 hours. After RT-PCR, the samples were electro-phoresised for expressing the MTC1-4 genes.Results: The expression of MCT 1-3 was observed in the first group, but the obtained results did not indicate their expression in the second group.Conclusion: Preservation of 4-cell embryos in 4oC for 24 hours inhibits the expression of MCT 1-3 genes. Keeping embryos in 4oC temperature is not a proper way for their short time preservation.

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APA: Copy

RAMEZANI, MINA, HOSSEINI, AHMAD, KAZEMI, BAHRAM, & JANAN, A.. (2010). THE EFFECT OF SHORT TIME EXPOSURE T TO 4^°C TEMPERATURE ON THE EXPRESSION PROFILES OF MONO-CARBOXYLIC TRANSPORTER GENES 1, 2, 3, AND 4 IN 4-CELL MOUSE EMBRYOS. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 13(2 (51)), 55-62. SID. https://sid.ir/paper/69514/en

Vancouver: Copy

RAMEZANI MINA, HOSSEINI AHMAD, KAZEMI BAHRAM, JANAN A.. THE EFFECT OF SHORT TIME EXPOSURE T TO 4^°C TEMPERATURE ON THE EXPRESSION PROFILES OF MONO-CARBOXYLIC TRANSPORTER GENES 1, 2, 3, AND 4 IN 4-CELL MOUSE EMBRYOS. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2010;13(2 (51)):55-62. Available from: https://sid.ir/paper/69514/en

IEEE: Copy

MINA RAMEZANI, AHMAD HOSSEINI, BAHRAM KAZEMI, and A. JANAN, “THE EFFECT OF SHORT TIME EXPOSURE T TO 4^°C TEMPERATURE ON THE EXPRESSION PROFILES OF MONO-CARBOXYLIC TRANSPORTER GENES 1, 2, 3, AND 4 IN 4-CELL MOUSE EMBRYOS,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 13, no. 2 (51), pp. 55–62, 2010, [Online]. Available: https://sid.ir/paper/69514/en

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