THE PROTECTIVE EFFECTS OF EPIGALLOCATECHIN GALLATE ON LIPOPOLYSA CCHARIDE-INDUCED HEPATOTOXICITY: AN IN VITRO STUDY ON HEP3B CELLS

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KARAMESE MURAT; GUVENDI BULENT; KARAMESE SELINA AKSAK; CINAR IRFAN; CAN SERPIL; EROL HUSEYIN SERKAN; AYDIN HAKAN; GELEN VOLKAN; KARAKUS EMRE

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Journal: Iranian Journal of Basic Medical Sciences Year:2016 | Volume:19 | Issue:5 Page(s): 483-489

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Title

THE PROTECTIVE EFFECTS OF EPIGALLOCATECHIN GALLATE ON LIPOPOLYSA CCHARIDE-INDUCED HEPATOTOXICITY: AN IN VITRO STUDY ON HEP3B CELLS

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Keywords

CYTOKINES

EPIGALLOCATECHIN GALLATE

HEP3B

LIPOPOLYSACCHARIDE

OXIDATIVE STRESS

Abstract

Objective (s): In the present study, our aim was to investigate the possible protective effects of EPIGALLOCATECHIN GALLATE (EGCG) on LIPOPOLYSACCHARIDE (LPS) -induced hepatotoxicity by using HEP3B human hepatoma cells. Specifically, the study examines the role of some proinflammatory markers and oxidative damage as possible mechanisms of LPS-associated cytotoxicity. Consequently, the hepatocellular carcinoma cell line HEP3B was chosen as a model for investigation of LPS toxicity and the effect of EGCG on LPS-exposed cells.Materials and Methods: The HEP3B human hepatoma cells were used for this study. The cytotoxic effects of chemicals (EGCG and LPS), AST and ALT levels, SOD and CAT activities, GSH-Px level and TNF-alpha and IL-6 levels were detected by using different biochemical and molecular methods. LPS and EGCG were applied to cells at various times and doses.Results: The highest treatment dose of EGCG (400 mM) led to a dramatic decrease in SOD level and increase in CAT and GSH levels. Additionally, the highest dose of EGCG also led to a dramatic increase in TNF-alpha and IL-6 levels. On the other hand, effective doses of EGCG (200 and 100 mM) normalized all related parameters levels.Conclusion: LPS caused hepatotoxicity, but interestingly, a high dose of EGCG was found to be a cytotoxic agent in this study. However, other two doses of EGCG led to a decrease in both inflammatory cytokine levels and antioxidant enzyme levels. Further studies should examine the effect of EGCG on secondary cellular signaling pathways.

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APA: Copy

KARAMESE, MURAT, GUVENDI, BULENT, KARAMESE, SELINA AKSAK, CINAR, IRFAN, CAN, SERPIL, EROL, HUSEYIN SERKAN, AYDIN, HAKAN, GELEN, VOLKAN, & KARAKUS, EMRE. (2016). THE PROTECTIVE EFFECTS OF EPIGALLOCATECHIN GALLATE ON LIPOPOLYSA CCHARIDE-INDUCED HEPATOTOXICITY: AN IN VITRO STUDY ON HEP3B CELLS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 19(5), 483-489. SID. https://sid.ir/paper/696188/en

Vancouver: Copy

KARAMESE MURAT, GUVENDI BULENT, KARAMESE SELINA AKSAK, CINAR IRFAN, CAN SERPIL, EROL HUSEYIN SERKAN, AYDIN HAKAN, GELEN VOLKAN, KARAKUS EMRE. THE PROTECTIVE EFFECTS OF EPIGALLOCATECHIN GALLATE ON LIPOPOLYSA CCHARIDE-INDUCED HEPATOTOXICITY: AN IN VITRO STUDY ON HEP3B CELLS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2016;19(5):483-489. Available from: https://sid.ir/paper/696188/en

IEEE: Copy

MURAT KARAMESE, BULENT GUVENDI, SELINA AKSAK KARAMESE, IRFAN CINAR, SERPIL CAN, HUSEYIN SERKAN EROL, HAKAN AYDIN, VOLKAN GELEN, and EMRE KARAKUS, “THE PROTECTIVE EFFECTS OF EPIGALLOCATECHIN GALLATE ON LIPOPOLYSA CCHARIDE-INDUCED HEPATOTOXICITY: AN IN VITRO STUDY ON HEP3B CELLS,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 19, no. 5, pp. 483–489, 2016, [Online]. Available: https://sid.ir/paper/696188/en

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