IDENTIFICATION AND CHARACTERIZATION OF COLON CANCER STEM CELLS IN HT-29 ADENOCARCINOMA CELL LINE

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KHORRAMI S.; ZAVARAN HOSSEINI A.; MOWLA J.; MALEKZADEH R.

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Journal: ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ) Year:2014 | Volume:17 | Issue:5 (86) Page(s): 21-30

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Information Journal Paper

Title

IDENTIFICATION AND CHARACTERIZATION OF COLON CANCER STEM CELLS IN HT-29 ADENOCARCINOMA CELL LINE

Author(s)

KHORRAMI S. | ZAVARAN HOSSEINI A. | MOWLA J. | MALEKZADEH R. | Issue Writer Certificate

Keywords

CANCER STEM CELLSQ2

CD44Q2

EPCAMQ2

HT-29Q2

SPHEROIDQ2

Abstract

Background: CANCER STEM CELLS are subpopulation of cancer cells that show self-renewal potential and the capacity to differentiate into diverse populations comprising a tumor. One of the characteristics of CSCs is their ability to form floating SPHEROIDs under anchorage-independent conditions in a serum-free media. The aim of this study was isolation of colon CANCER STEM CELLS by sphere formation assay and characterization of them in human colonic adenocarcinoma HT-29.Materials and Methods: In this experimental study, colon CSCs markers including CD44 and EPCAM in SPHEROID and HT-29 cells were analyzed by flow cytometry. The expression levels of stemness genes in both SPHEROID and HT-29 cells were investigated using real-time PCR. Tumorigenic potential of SPHEROID cells was evaluated usingin vivo xenografts assay.Results: Our data showed over 92% of SPHEROIDs were CD44+/EpCAM+, while HT-29 cells only have expressed 37% of CD44/EpCAM markers. In compared with the HT-29 cells, expression levels of ‘‘stemness’’ genes, like Sox2, Oct4, Nanog, C-myc, and Klf4 were significantly increased in SPHEROID cells (p<0.05). Further, As little as 2500 SPHEROID cells were sufficient to obtain tumor growth in nude mice, while 1x106 of HT-29 cells was needed to form tumor.Conclusion: Our data suggest that SPHEROID formed by colon cancer cell lines highly enriched in CSCs and showed increasing expression of stemness genes and tumorigenic in nude mice.

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APA: Copy

KHORRAMI, S., ZAVARAN HOSSEINI, A., MOWLA, J., & MALEKZADEH, R.. (2014). IDENTIFICATION AND CHARACTERIZATION OF COLON CANCER STEM CELLS IN HT-29 ADENOCARCINOMA CELL LINE. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 17(5 (86)), 21-30. SID. https://sid.ir/paper/69850/en

Vancouver: Copy

KHORRAMI S., ZAVARAN HOSSEINI A., MOWLA J., MALEKZADEH R.. IDENTIFICATION AND CHARACTERIZATION OF COLON CANCER STEM CELLS IN HT-29 ADENOCARCINOMA CELL LINE. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2014;17(5 (86)):21-30. Available from: https://sid.ir/paper/69850/en

IEEE: Copy

S. KHORRAMI, A. ZAVARAN HOSSEINI, J. MOWLA, and R. MALEKZADEH, “IDENTIFICATION AND CHARACTERIZATION OF COLON CANCER STEM CELLS IN HT-29 ADENOCARCINOMA CELL LINE,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 17, no. 5 (86), pp. 21–30, 2014, [Online]. Available: https://sid.ir/paper/69850/en

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