DOWNREGULATING THE EXPRESSION OF CHID1 BY CHITIN MICROPARTICLES MIXED LEUKOCYTE CULTURE

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ALIMOHAMMADI MASUMEH; YEGANEH FARSHID; HAJI MOLLA HOSEINI MOSTAFA

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Journal: ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ) Year:2016 | Volume:19 | Issue:4 (109) Page(s): 42-50

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Title

DOWNREGULATING THE EXPRESSION OF CHID1 BY CHITIN MICROPARTICLES MIXED LEUKOCYTE CULTURE

Author(s)

ALIMOHAMMADI MASUMEH | YEGANEH FARSHID | HAJI MOLLA HOSEINI MOSTAFA | Issue Writer Certificate

Keywords

CHITINQ1

CHITINASEQ1

CHITOSANQ1

IMMUNE MODULATIONQ1

Abstract

Background: CHITIN and its derivates microparticles (MPs) have immunomodulatory activities. In this study, we examined the effect of size, purity and acetylation degree of CHITIN MPs on CHID1- encoding SI-CLP, involved in inflammation- gene expression in mixed leukocyte culture.Materials and Methods: Small (<40) and medium(40-70 m) sized CHITIN MPs were prepared by sonication, and they were used in treatment of leukocyte mixed culture in comparison with CHITOSAN and also shrimp shell small-sized MPs. Neutral red uptake assay and microscopic examination of apoptosis were used to assess cytotoxicity of MPs. Finally, following cell treatment with MPs (100 mg/mL) for 48h, expression levels of CHID1 gene were determined by Real Time PCR.results: Different concentrations of CHITINous MPs hadn’t any cytotoxic effects. In gene expression analysis, small-sized CHITIN MPs (<40 m) resulted in down regulation of CHID1 gene expression (p=0.004), while other MPs didn’t change it significantly.Conclusion: Size, purity and acetylation degree of CHITIN MPs influence their interference in immune cells interactions and it seems small-sized CHITIN MPs can potentially modulate immune responses through decreasing CHID1 gene expression. Using small-sized CHITIN MPs may be effective to treat allergies which their treatment strategies rely on modulating the immune responses.

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APA: Copy

ALIMOHAMMADI, MASUMEH, YEGANEH, FARSHID, & HAJI MOLLA HOSEINI, MOSTAFA. (2016). DOWNREGULATING THE EXPRESSION OF CHID1 BY CHITIN MICROPARTICLES MIXED LEUKOCYTE CULTURE. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 19(4 (109)), 42-50. SID. https://sid.ir/paper/69896/en

Vancouver: Copy

ALIMOHAMMADI MASUMEH, YEGANEH FARSHID, HAJI MOLLA HOSEINI MOSTAFA. DOWNREGULATING THE EXPRESSION OF CHID1 BY CHITIN MICROPARTICLES MIXED LEUKOCYTE CULTURE. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2016;19(4 (109)):42-50. Available from: https://sid.ir/paper/69896/en

IEEE: Copy

MASUMEH ALIMOHAMMADI, FARSHID YEGANEH, and MOSTAFA HAJI MOLLA HOSEINI, “DOWNREGULATING THE EXPRESSION OF CHID1 BY CHITIN MICROPARTICLES MIXED LEUKOCYTE CULTURE,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 19, no. 4 (109), pp. 42–50, 2016, [Online]. Available: https://sid.ir/paper/69896/en

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