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Title

IN VITRO EFFECTS OF WISTAR RAT PRENATAL AND POSTNATAL CEREBROSPINAL FLUID ON NEURAL DIFFERENTIATION AND P ROLIFERATION OF MESENCHYMAL STROMAL CELLS DERIVED FROM BONE MARROW

Pages

  537-544

Abstract

 Objective: CEREBROSPINAL FLUID (CSF) plays an important role in cortical development during the fetal stages. Embryonic CSF (E-CSF) consists of numerous neurotrophic and growth factors that regulate neurogenesis, differentiation, and proliferation. Mesenchymal stem cells (MSCs) are multi-potential stem cells that can differentiate into mesenchymal and non-mesenchymal cells, including neural cells. This study evaluates the prenatal and postnatal effects of CSF on proliferation and NEURAL DIFFERENTIATION of bone marrow MSCs (BM-MSCs) at gestational ages E19, E20, and the first day after birth (P1).Materials and Methods: In this experimental study, we confirmed the mesenchymal nature of BM-MSCs according to their adherence properties and surface markers (CD44, CD73 and CD45). The multi-potential characteristics of BMMSCs were verified by assessments of the osteogenic and adipogenic potentials of these cells. Under appropriatein vitroconditions, the BM-MSCs cultures were incubated with and without additional pre- and postnatal CSF. The MTT assay was used to quantify cellular proliferation and viability. Immunocytochemistry was used to study the expression of MAP-2 and β-III tubulin in the BM-MSCs. We used ImageJ software to measure the length of the neurites in the cultured cells.Results: BM-MSCs differentiated into neuronal cell types when exposed to basic fibroblast growth factor (b-FGF).Viability and proliferation of the BM-MSCs conditioned with E19, E20, and P1 CSF increased compared to the control group. We observed significantly elevated NEURAL DIFFERENTIATION of the BM-MSCS cultured in the CSF-supplemented medium from E19 compared to cultures conditioned with E20 and P1 CSF group.Conclusion: The results have confirmed that E19, E20, and P1 CSF could induce proliferation and differentiation of BM-MSCs though they are age dependent factors. The presented data support a significant, conductive role of CSF components in neuronal survival, proliferation, and differentiation.

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    APA: Copy

    SHOKOHI, ROZMEHR, NABIUNI, MOHAMMAD, IRIAN, SAEED, & MIYAN, JALEEL A.. (2018). IN VITRO EFFECTS OF WISTAR RAT PRENATAL AND POSTNATAL CEREBROSPINAL FLUID ON NEURAL DIFFERENTIATION AND P ROLIFERATION OF MESENCHYMAL STROMAL CELLS DERIVED FROM BONE MARROW. CELL JOURNAL (YAKHTEH), 19(4), 537-544. SID. https://sid.ir/paper/699666/en

    Vancouver: Copy

    SHOKOHI ROZMEHR, NABIUNI MOHAMMAD, IRIAN SAEED, MIYAN JALEEL A.. IN VITRO EFFECTS OF WISTAR RAT PRENATAL AND POSTNATAL CEREBROSPINAL FLUID ON NEURAL DIFFERENTIATION AND P ROLIFERATION OF MESENCHYMAL STROMAL CELLS DERIVED FROM BONE MARROW. CELL JOURNAL (YAKHTEH)[Internet]. 2018;19(4):537-544. Available from: https://sid.ir/paper/699666/en

    IEEE: Copy

    ROZMEHR SHOKOHI, MOHAMMAD NABIUNI, SAEED IRIAN, and JALEEL A. MIYAN, “IN VITRO EFFECTS OF WISTAR RAT PRENATAL AND POSTNATAL CEREBROSPINAL FLUID ON NEURAL DIFFERENTIATION AND P ROLIFERATION OF MESENCHYMAL STROMAL CELLS DERIVED FROM BONE MARROW,” CELL JOURNAL (YAKHTEH), vol. 19, no. 4, pp. 537–544, 2018, [Online]. Available: https://sid.ir/paper/699666/en

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