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Information Journal Paper

Title

LEPTIN IMPROVES SPERM CRYOPRESERVATION VIA ANTIOXIDANT DEFENSE

Pages

  172-178

Abstract

 Background: LEPTIN and its receptor are present in SPERMatozoa; however, the role of LEPTIN in SPERM function is still controversial. Our present study aimed at demonstrating the effect of cryopreservation on SPERM DNA FRAGMENTATION (DNAf) and investigating the possible effects of SPERM capacitation techniques and LEPTINin vitro incubation on frozen-thawed SPERM DNAf and OXIDATIVE STRESS.Methods: Samples of 45 normospermic men attending for infertility investigation at Vida Centro de Fertilidade, Rio de Janeiro, Brazil, were frozen and thawed with or without capacitation and LEPTIN incubation prior to freezing. SPERM DNA FRAGMENTATION was evaluated by SPERM Chromatin Dispersion Assay before and after cryopreservation and OXIDATIVE STRESS parameters were measured by spectrophotometry with and without LEPTIN incubation. Statistical analysis was performed using paired t test to compare DNAf between groups before and after FREEZE-THAW CYCLE, to compare groups before and after capacitation and LEPTIN incubation and oxidative measurements before and after LEPTIN incubation. Statistical significance was considered when p≤0.05.Results: Our results revealed a significant post-thaw rise in SPERM DNAf compared with fresh samples (p=0.0003). SPERM DNAf was significantly reduced when SPERM capacitation was performed before freezing, when compared to those frozen with no previous capacitation (p=0.01). The addition of LEPTIN to capacitated SPERM before freezing reduced DNAf (p<0.0001) and enhanced superoxide dismutase (p=0.001) and glutathione peroxidase (p=0.02) antioxidant enzymes activity.Conclusion: The addition of LEPTIN to capacitated SPERM can improve SPERM DNA quality following cryopreservation, possibly by inducing the activity of certain antioxidant enzymes.

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    APA: Copy

    FONTOURA, PAULA, MELLO, MARIANA DUQUE, GALLO SA, PAULO, ERTHAL MARTINS, MARIA CECILIA, ALMEIDA CARDOSO, MARIA CECILIA, & RAMOS, CRISTIANE. (2017). LEPTIN IMPROVES SPERM CRYOPRESERVATION VIA ANTIOXIDANT DEFENSE. JOURNAL OF REPRODUCTION AND INFERTILITY, 18(1), 172-178. SID. https://sid.ir/paper/700303/en

    Vancouver: Copy

    FONTOURA PAULA, MELLO MARIANA DUQUE, GALLO SA PAULO, ERTHAL MARTINS MARIA CECILIA, ALMEIDA CARDOSO MARIA CECILIA, RAMOS CRISTIANE. LEPTIN IMPROVES SPERM CRYOPRESERVATION VIA ANTIOXIDANT DEFENSE. JOURNAL OF REPRODUCTION AND INFERTILITY[Internet]. 2017;18(1):172-178. Available from: https://sid.ir/paper/700303/en

    IEEE: Copy

    PAULA FONTOURA, MARIANA DUQUE MELLO, PAULO GALLO SA, MARIA CECILIA ERTHAL MARTINS, MARIA CECILIA ALMEIDA CARDOSO, and CRISTIANE RAMOS, “LEPTIN IMPROVES SPERM CRYOPRESERVATION VIA ANTIOXIDANT DEFENSE,” JOURNAL OF REPRODUCTION AND INFERTILITY, vol. 18, no. 1, pp. 172–178, 2017, [Online]. Available: https://sid.ir/paper/700303/en

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