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Information Journal Paper

Title

Genome-based Detection of Novel Coronavirus: An Overview Study

Pages

  51-60

Keywords

Coronavirus Disease 2019 (COVID-19) 

Abstract

 The Coronavirus Disease 2019, named COVID-19 is a global problem. According to the declaration of the World Health Organization (WHO), it is a novel and extreme Outbreak, spreading worldwide. Firstly, numerous patients reported exposure in Wuhan City, China at a large animals and seafood market. Accordingly, the first idea is suggesting the animal-to-human transmission of this infection pathogen. Next, since then, numerous patients have decelerated no exposure to animal shops, indicating that human-to-human transmission is occurring. The new Coronavirus (2019-nCoV) is a positive RNA virus with a 29. 8 kb genome and S, E, M, N, and Orf1 gene fragments. The most popular method for 2019-nCoV Detection is genomebased approaches, like Polymerase Chain Reaction (PCR)-based tests that require expensive experimental equipment, a controlled working environment, and high-trained technicians; they are often lacking in massive viral Outbreaks. Therefore, another rapid and simple genome-based assay was evaluated for this pathogen. In this review study, the RT-LAMP technique, as the main Isothermal amplification assays with less time consumption and without the need for expensive equipment, compared to conventional PCR-based methods to 2019-nCoV identification, was discussed. Accordingly, the advantage or disadvantage of these techniques was compared. The obtained data indicated that this molecular and isothermal method could be used as a successful one-step process for portable screening and the rapid identification of 2019-nCoV.

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    Cite

    APA: Copy

    ZEINODDINI, MEHDI. (2021). Genome-based Detection of Novel Coronavirus: An Overview Study. JOURNAL OF INFLAMMATORY DISEASES (THE JOURNAL OF QAZVIN UNIVERSITY OF MEDICAL SCIENCES), 25(1 (114) ), 51-60. SID. https://sid.ir/paper/700504/en

    Vancouver: Copy

    ZEINODDINI MEHDI. Genome-based Detection of Novel Coronavirus: An Overview Study. JOURNAL OF INFLAMMATORY DISEASES (THE JOURNAL OF QAZVIN UNIVERSITY OF MEDICAL SCIENCES)[Internet]. 2021;25(1 (114) ):51-60. Available from: https://sid.ir/paper/700504/en

    IEEE: Copy

    MEHDI ZEINODDINI, “Genome-based Detection of Novel Coronavirus: An Overview Study,” JOURNAL OF INFLAMMATORY DISEASES (THE JOURNAL OF QAZVIN UNIVERSITY OF MEDICAL SCIENCES), vol. 25, no. 1 (114) , pp. 51–60, 2021, [Online]. Available: https://sid.ir/paper/700504/en

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