IMMOBILIZATION OF THE ALKALINE PHOSPHATASE ON COLLAGEN SURFACE VIA CROSS-LINKING METHOD

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HANACHI PARICHEHR; JAFARI FARZANEH; JAFARI FARIBA; Motamedi Shima

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Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF BIOTECHNOLOGY Year:2015 | Volume:13 | Issue:3 Page(s): 32-38

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Title

IMMOBILIZATION OF THE ALKALINE PHOSPHATASE ON COLLAGEN SURFACE VIA CROSS-LINKING METHOD

Author(s)

HANACHI PARICHEHR | JAFARI FARZANEH | JAFARI FARIBA | Motamedi Shima

Keywords

ALKALINE PHOSPHATASE

ENZYME ACTIVITY

IMMOBILIZATION

MATRIX

Abstract

Background: Collagen, the most abundant protein in the human body, and as an extracellular matrix protein, has an importantrole in the fiber formation. This feature of the collagen renders establishment of the structural skeleton in tissues.Regarding specific features associated with the collagen, such as, formation of the porous structure, permeability andhydrophilicity, it can also be used as a biocompatible matrix in the enzyme engineering.Objectives: The aim of the present study was to investigate the application of the type I collagen as a matrix for alkalinephosphatase immobilization using cross-linking method.Material and Methods: The Alkaline phosphatase was covalently immobilized on collagen matrix by using 1-ethyl-3-(dimethylaminopropyl) carbodiimide hydrochloride (EDC). The source of the alkaline phosphatase was from the bovineintestinal mucous. After that, the activity of the immobilized enzyme was assayed under different experimental conditions.Results: The optimum pH was similar to that of the free enzyme, whereas the optimum temperature and thermal stabilitywere shown some increments. The surface topography of the collagen matrix containing immobilized enzyme and ALP(Alkaline phosphatase) deficient was investigated by Atomic-force microscopy (AFM). Images that have been obtainedapplying AFM show significant differences between uncovered and immobilized enzyme- matrix surface topography.Conclusions: Our findings suggest that type I collagen can be utilized as a matrix for alkaline phosphatase immobilizationvia cross-linking method.

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APA: Copy

HANACHI, PARICHEHR, JAFARI, FARZANEH, JAFARI, FARIBA, & Motamedi, Shima. (2015). IMMOBILIZATION OF THE ALKALINE PHOSPHATASE ON COLLAGEN SURFACE VIA CROSS-LINKING METHOD. IRANIAN JOURNAL OF BIOTECHNOLOGY, 13(3), 32-38. SID. https://sid.ir/paper/701446/en

Vancouver: Copy

HANACHI PARICHEHR, JAFARI FARZANEH, JAFARI FARIBA, Motamedi Shima. IMMOBILIZATION OF THE ALKALINE PHOSPHATASE ON COLLAGEN SURFACE VIA CROSS-LINKING METHOD. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2015;13(3):32-38. Available from: https://sid.ir/paper/701446/en

IEEE: Copy

PARICHEHR HANACHI, FARZANEH JAFARI, FARIBA JAFARI, and Shima Motamedi, “IMMOBILIZATION OF THE ALKALINE PHOSPHATASE ON COLLAGEN SURFACE VIA CROSS-LINKING METHOD,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 13, no. 3, pp. 32–38, 2015, [Online]. Available: https://sid.ir/paper/701446/en

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