Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

521
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

115
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

VITRIFICATION OF MOUSE MII OOCYTE DECREASES THE MITOCHONDRIAL DNA COPY NUMBER, TFAM GENE EXPRESSION AND MITOCHONDRIAL ENZYME ACTIVITY

Pages

  343-351

Abstract

 Background: The objective of this study was determination of the changes in the REACTIVE OXYGEN SPECIES (ROS) level, mitochondrial DNA (mtDNA) copy number and enzyme activity and transcription factor A (TFAM) gene expression in oocytes after vitrification.Methods: The oocytes at metaphase II (MII) stage (n=320) were collected from superovulated adult female mice (n=40). These oocytes were divided into vitrified and non-vitrified groups (n=160 in each group). After vitrification of oocytes, ROS level, MTDNA COPY NUMBER; TFAM gene expression and mitochondrial enzymes activity (CYTOCHROME C OXIDASE and SUCCINATE DEHYDROGENASE) were assessed and compared with non-vitrified group. Visualization of the mitochondria was done using Mitotracker green staining under confocal microscope. Data were compared by independent T-test. Values of p<0.05 were considered as statistically significant.Results: The survival rate of oocytes after vitrification and warming was 96.05%.The intensity of CYTOCHROME C OXIDASE activity, MTDNA COPY NUMBER and TFAM gene expression in non-vitrified oocytes were significantly lower and the level of ROS was higher in vitrified oocytes in comparison with non-vitrified group (p<0.05). But the intensity of SUCCINATE DEHYDROGENASE activity was not significantly different between the two groups. The pattern of mitochondrial distribution in two groups of study was similar but the intensity of Mitotracker green in non-vitrified oocytes was significantly higher than vitrified oocytes (p<0.05).Conclusion: This study showed that vitrification of mouse MII oocytes reduced the MTDNA COPY NUMBER and mitochondrial CYTOCHROME C OXIDASE activity by increasing ROS level, thus the subsequent embryo development may be affected.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    AMOUSHAHI, MAHBOOBEH, SALEHNIA, MOJDEH, & MOWLA, SEYED JAVAD. (2017). VITRIFICATION OF MOUSE MII OOCYTE DECREASES THE MITOCHONDRIAL DNA COPY NUMBER, TFAM GENE EXPRESSION AND MITOCHONDRIAL ENZYME ACTIVITY. JOURNAL OF REPRODUCTION AND INFERTILITY, 18(4), 343-351. SID. https://sid.ir/paper/701768/en

    Vancouver: Copy

    AMOUSHAHI MAHBOOBEH, SALEHNIA MOJDEH, MOWLA SEYED JAVAD. VITRIFICATION OF MOUSE MII OOCYTE DECREASES THE MITOCHONDRIAL DNA COPY NUMBER, TFAM GENE EXPRESSION AND MITOCHONDRIAL ENZYME ACTIVITY. JOURNAL OF REPRODUCTION AND INFERTILITY[Internet]. 2017;18(4):343-351. Available from: https://sid.ir/paper/701768/en

    IEEE: Copy

    MAHBOOBEH AMOUSHAHI, MOJDEH SALEHNIA, and SEYED JAVAD MOWLA, “VITRIFICATION OF MOUSE MII OOCYTE DECREASES THE MITOCHONDRIAL DNA COPY NUMBER, TFAM GENE EXPRESSION AND MITOCHONDRIAL ENZYME ACTIVITY,” JOURNAL OF REPRODUCTION AND INFERTILITY, vol. 18, no. 4, pp. 343–351, 2017, [Online]. Available: https://sid.ir/paper/701768/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top