مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

100
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

49
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Optimization of Production Conditions for Bioactive Polysaccharides from Fomes fomentarius and Investigation of Antibacterial and Antitumor Activities

Pages

  596-602

Abstract

 Background: One of the medicinal fungi that has been used in traditional medicine for a long time is the Basidiomycete fungus Fomes fomentarius, which is widely distributed in Iran. Polysaccharides as one of the metabolites of this fungus have anti-inflammatory, anti-diabetic, Antibacterial, Antioxidant, and anti-cancer properties. Materials & Methods: Optimization of independent variables of MgSO4. 7H2O concentration, initial pH, yeast extract, and inoculum percentage to increase biomass and polysaccharide production of F. fomentarius was investigated using the Taguchi method. Then, the biological properties of the produced polysaccharide including Antibacterial activity was investigated by bacterial colony counting method, Antioxidant activity using DPPH free radical, and antiproliferative effect on 5 cancer cell lines MKN-45, AGS, A549, KYSE-30 and 5637 using MTS test. Results: The concentration of MgSO4. 7H2O and initial pH had a significant effect (P<0. 05) on the production of F. fomentarius polysaccharide and in optimal conditions polysaccharide production reaches 5. 410 g/L. The polysaccharide of this fungus inhibits the growth of Staphylococcus aureus and Escherichia coli bacteria by 50% and 25%, respectively. The Antioxidant activity of this polysaccharide in the DPPH test is 16. 11%. The antiproliferative effect of this polysaccharide on cancer cells is different (KYSE-30> A549 ≥ 5637> AGS> MKN-45). This effect increases with increasing concentration. In KYSE-30 cell line treatment with 200 g/mL polysaccharide, cell viability reaches 40% after 72 hours. Conclusion: Optimizing the culture medium of the medicinal fungus Fomes fomentarius increases the production of polysaccharides up to 5. 410 g/L. Optimization increases the biological activity of polysaccharides. Antibacterial activity against Staphylococcus aureus and Escherichia coli is 50% and 25%, respectively. The Antioxidant activity of polysaccharides is 16. 11% and the viability of KYSE-30 cancer cells reaches 40% after 72 hours.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    Alvandi, Hale, HATAMIAN ZARMI, ASHRAFALSADAT, Ebrahimi hosseinzadeh, Bahman, & Beagom Mokhtari Hosseini, Zahra. (2020). Optimization of Production Conditions for Bioactive Polysaccharides from Fomes fomentarius and Investigation of Antibacterial and Antitumor Activities. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, 14(6 ), 596-602. SID. https://sid.ir/paper/701933/en

    Vancouver: Copy

    Alvandi Hale, HATAMIAN ZARMI ASHRAFALSADAT, Ebrahimi hosseinzadeh Bahman, Beagom Mokhtari Hosseini Zahra. Optimization of Production Conditions for Bioactive Polysaccharides from Fomes fomentarius and Investigation of Antibacterial and Antitumor Activities. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY[Internet]. 2020;14(6 ):596-602. Available from: https://sid.ir/paper/701933/en

    IEEE: Copy

    Hale Alvandi, ASHRAFALSADAT HATAMIAN ZARMI, Bahman Ebrahimi hosseinzadeh, and Zahra Beagom Mokhtari Hosseini, “Optimization of Production Conditions for Bioactive Polysaccharides from Fomes fomentarius and Investigation of Antibacterial and Antitumor Activities,” IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, vol. 14, no. 6 , pp. 596–602, 2020, [Online]. Available: https://sid.ir/paper/701933/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button