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Information Journal Paper

Title

DI-(2-ETHYLHEXYL) PHTHALATE-INDUCED HIPPOCAMPUS-DERIVED NEURAL STEM CELLS PROLIFERATION

Pages

  166-172

Abstract

 The brain and spinal cord have a limited capacity for self-repair under damaged conditions.One of the best options to overcome these limitations involves the use of phytochemicals as potential therapeutic agents. In this study, we have aimed to investigate the effects of di- (2-ethylhexyl) phthalate (DEHP) on hippocampus-derived NEURAL STEM CELLS (NSCs) PROLIFERATION to search phytochemical candidates for possible treatment of neurological diseases using endogenous capacity.In this experimental study, neonatal rat hippocampus-derived NSCs were cultured and treated with various concentrations of DEHP (0, 100, 200, 400 and 600 μM) andCirsium vulgare (C. vulgare)hydroethanolic extract (0, 200, 400, 600, 800 and 1000 μg/ml) for 48 hours underin vitro conditions. Cell PROLIFERATION rates and quantitative SOX2 gene expression were evaluated using MTT assay and real-time reverse transcription polymerase chain reaction (RT-PCR).We observed the highest average growth rate in the 400 μM DEHP and 800 μg/mlC.vulgareextract treated groups. SOX2 expression in the DEHP-treated NSCs significantly increased compared to the control group. Gas chromatography/mass spectrometry (GC/ MS) results demonstrated that the active ingredients that naturally occurred in theC. vulgare hydroethanolic extract were 2-ethyl-1-hexanamine, n-heptacosane, 1-cyclopentanecarboxylic acid, 1-heptadecanamine, 2, 6-octadien-1-ol, 2, 6, 10, 14, 18, 22-tetracosahexaene, and DEHP. DEHP profoundly stimulated NSCs PROLIFERATION throughSox2 gene overexpression.These results provide and opportunity for further use of theC. vulgure phytochemicals for prevention and/or treatment of neurological diseases via phytochemical mediated-PROLIFERATION of endogenous adult NSCs.

Cites

References

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APA: Copy

ABDANIPOUR, ALIREZA, NOORI ZADEH, ALI, MESBAH NAMIN, SEYED ALIREZA, BAKHTIYARI, SALAR, NEJATBAKHSH, REZA, & JAFARI ANARKOOLI, IRAJ. (2017). DI-(2-ETHYLHEXYL) PHTHALATE-INDUCED HIPPOCAMPUS-DERIVED NEURAL STEM CELLS PROLIFERATION. CELL JOURNAL (YAKHTEH), 19(1), 166-172. SID. https://sid.ir/paper/703395/en

Vancouver: Copy

ABDANIPOUR ALIREZA, NOORI ZADEH ALI, MESBAH NAMIN SEYED ALIREZA, BAKHTIYARI SALAR, NEJATBAKHSH REZA, JAFARI ANARKOOLI IRAJ. DI-(2-ETHYLHEXYL) PHTHALATE-INDUCED HIPPOCAMPUS-DERIVED NEURAL STEM CELLS PROLIFERATION. CELL JOURNAL (YAKHTEH)[Internet]. 2017;19(1):166-172. Available from: https://sid.ir/paper/703395/en

IEEE: Copy

ALIREZA ABDANIPOUR, ALI NOORI ZADEH, SEYED ALIREZA MESBAH NAMIN, SALAR BAKHTIYARI, REZA NEJATBAKHSH, and IRAJ JAFARI ANARKOOLI, “DI-(2-ETHYLHEXYL) PHTHALATE-INDUCED HIPPOCAMPUS-DERIVED NEURAL STEM CELLS PROLIFERATION,” CELL JOURNAL (YAKHTEH), vol. 19, no. 1, pp. 166–172, 2017, [Online]. Available: https://sid.ir/paper/703395/en

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