PHIC31-BASED SITE-SPECIFIC TRANSGENESIS SYSTEM FOR PRODUCTION OF TRANSGENIC BOVINE EMBRYOS BY SOMATIC CELL NUCLEAR TRANSFER AND INTRACYTOPLASMIC SPERM INJECTION

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SEKHAVATI MOHAMMAD HADI; HOSSEINI SAYED MORTEZA; TAHMOORESPUR MOJTABA; GHAEDI KAMRAN; JAFARPOUR FARNOOSH; HAJIAN MEHDI; DORMIANI KYANOOSH; NASR ESFAHANI MOHAMMAD HOSSAIN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2018 | Volume:20 | Issue:1 Page(s): 98-107

Download Full-Text

View:

282

Download:

170

Cites:

Information Journal Paper

Title

PHIC31-BASED SITE-SPECIFIC TRANSGENESIS SYSTEM FOR PRODUCTION OF TRANSGENIC BOVINE EMBRYOS BY SOMATIC CELL NUCLEAR TRANSFER AND INTRACYTOPLASMIC SPERM INJECTION

Author(s)

Keywords

INTRACYTOPLASMIC SPERM INJECTION

SOMATIC CELL NUCLEAR TRANSFER

TRANSGENESIS

Abstract

Objective: The Streptomyces phage phiC31 integrase offers a sequence-specific method of TRANSGENESIS with a robust long-term gene expression. PhiC31 has been successfully developed in a variety of tissues and organs for purpose ofin vivo gene therapy. The objective of the present experiment was to evaluate PhiC31-based site-specific TRANSGENESIS system for production of transgenic bovine embryos by SOMATIC CELL NUCLEAR TRANSFER and INTRACYTOPLASMIC SPERM INJECTION.Materials and Methods: In this experimental study, the application of phiC31 integrase system was evaluated for generating transgenic bovine embryos by SOMATIC CELL NUCLEAR TRANSFER (SCNT) and sperm mediated gene transfer (SMGT) approaches.Results: PhiC31 integrase mRNA and protein was produced in vitro and their functionality was confirmed. Seven phiC31 recognizable bovine pseudo attachment sites of phage (attP) sites were considered for evaluation of site specific recombination. The accuracy of these sites was validated in phic31 targeted bovine fibroblasts using polymerase chain reaction (PCR) and sequencing. The efficiency and site-specificity of phiC31 integrase system was also confirmed in generated transgenic bovine embryo which successfully obtained using SCNT and SMGT technique.Conclusion: The results showed that both SMGT and SCNT-derived embryos were enhanced green fluorescent protein (EGFP) positive and phiC31 integrase could recombine the reporter gene in a site specific manner. These results demonstrate that attP site can be used as a proper location to conduct site directed TRANSGENESIS in both mammalian cells and embryos in phiC31 integrase system when even combinaed to SCNT and intracytoplasmic sperminjection (ICSI) method.

Cites

The Epigenetic Regulation of Blinatumomab Gene Expression: Tumor Cell-dependent T cell Response against Lymphoma Cells and Cytotoxic Activity

References

No record.

Cite

APA: Copy

SEKHAVATI, MOHAMMAD HADI, HOSSEINI, SAYED MORTEZA, TAHMOORESPUR, MOJTABA, GHAEDI, KAMRAN, JAFARPOUR, FARNOOSH, HAJIAN, MEHDI, DORMIANI, KYANOOSH, & NASR ESFAHANI, MOHAMMAD HOSSAIN. (2018). PHIC31-BASED SITE-SPECIFIC TRANSGENESIS SYSTEM FOR PRODUCTION OF TRANSGENIC BOVINE EMBRYOS BY SOMATIC CELL NUCLEAR TRANSFER AND INTRACYTOPLASMIC SPERM INJECTION. CELL JOURNAL (YAKHTEH), 20(1), 98-107. SID. https://sid.ir/paper/708559/en

Vancouver: Copy

SEKHAVATI MOHAMMAD HADI, HOSSEINI SAYED MORTEZA, TAHMOORESPUR MOJTABA, GHAEDI KAMRAN, JAFARPOUR FARNOOSH, HAJIAN MEHDI, DORMIANI KYANOOSH, NASR ESFAHANI MOHAMMAD HOSSAIN. PHIC31-BASED SITE-SPECIFIC TRANSGENESIS SYSTEM FOR PRODUCTION OF TRANSGENIC BOVINE EMBRYOS BY SOMATIC CELL NUCLEAR TRANSFER AND INTRACYTOPLASMIC SPERM INJECTION. CELL JOURNAL (YAKHTEH)[Internet]. 2018;20(1):98-107. Available from: https://sid.ir/paper/708559/en

IEEE: Copy

MOHAMMAD HADI SEKHAVATI, SAYED MORTEZA HOSSEINI, MOJTABA TAHMOORESPUR, KAMRAN GHAEDI, FARNOOSH JAFARPOUR, MEHDI HAJIAN, KYANOOSH DORMIANI, and MOHAMMAD HOSSAIN NASR ESFAHANI, “PHIC31-BASED SITE-SPECIFIC TRANSGENESIS SYSTEM FOR PRODUCTION OF TRANSGENIC BOVINE EMBRYOS BY SOMATIC CELL NUCLEAR TRANSFER AND INTRACYTOPLASMIC SPERM INJECTION,” CELL JOURNAL (YAKHTEH), vol. 20, no. 1, pp. 98–107, 2018, [Online]. Available: https://sid.ir/paper/708559/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops