INDIRECT CO-CULTURE OF TESTICULAR CELLS WITH BONE MARROW MESENCHYMAL STEM CELLS LEADS TO MALE GERM CELL-SPECIFIC GENE EXPRESSIONS

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GHORBANLOU MEHRDAD; ABDANIPOUR ALIREZA; SHIRAZI REZA; MALEKMOHAMMADI NASIM; SHOKRI SAEED; NEJATBAKHSH REZA

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Journal: Cell Journal (Yakhteh) Year:2019 | Volume:20 | Issue:4 Page(s): 505-512

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Title

INDIRECT CO-CULTURE OF TESTICULAR CELLS WITH BONE MARROW MESENCHYMAL STEM CELLS LEADS TO MALE GERM CELL-SPECIFIC GENE EXPRESSIONS

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Keywords

CO-CULTURE

GERM CELLS

MESENCHYMAL STEM CELLS

RETINOIC ACID

TESTIS

Abstract

Objective: Non-obstructive azoospermia is mostly irreversible. Efforts to cure this type of infertility have led to the application of stem cells in the reproduction field. In the present study, testicular cell-mediated differentiation of male germ-like cells from bone marrow-derived MESENCHYMAL STEM CELLS (BM-MSCs) in anin vitro indirect CO-CULTURE system is investigated.Materials and Methods: In this experimental study, mouse BM-MSCs were isolated and cultured up to passage three. Identification of the cells was evaluated using specific surface markers by flow-cytometry technique. Four experimental groups were investigated: control, treatment with RETINOIC ACID (RA), indirect CO-CULTURE with testicular cells, and combination of RA and indirect CO-CULTURE with testicular cells. Finally, following differentiation, the quantitative expression of germ cell-specific markers includingDazl, Piwil2 and Stra8 were evaluated by real-time polymerase chain reaction (PCR).Results: Molecular analysis revealed a significant increase in Dazl expression in the indirect CO-CULTURE with testicular cells group in comparison to the control group. Quantitative expression level ofPiwil2 was not significantly changed in comparison to the control group.Stra8 expression was significantly higher in RA group in comparison to other groups.Conclusion: Indirect CO-CULTURE of BM-MSCs in the presence of testicular cells leads to expression of male germ cell-specific gene, Dazl, in the induced cells. Combination of CO-CULTURE with testicular cells and RA did not show any positive effect on the specific gene expressions.

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APA: Copy

GHORBANLOU, MEHRDAD, ABDANIPOUR, ALIREZA, SHIRAZI, REZA, MALEKMOHAMMADI, NASIM, SHOKRI, SAEED, & NEJATBAKHSH, REZA. (2019). INDIRECT CO-CULTURE OF TESTICULAR CELLS WITH BONE MARROW MESENCHYMAL STEM CELLS LEADS TO MALE GERM CELL-SPECIFIC GENE EXPRESSIONS. CELL JOURNAL (YAKHTEH), 20(4), 505-512. SID. https://sid.ir/paper/708966/en

Vancouver: Copy

GHORBANLOU MEHRDAD, ABDANIPOUR ALIREZA, SHIRAZI REZA, MALEKMOHAMMADI NASIM, SHOKRI SAEED, NEJATBAKHSH REZA. INDIRECT CO-CULTURE OF TESTICULAR CELLS WITH BONE MARROW MESENCHYMAL STEM CELLS LEADS TO MALE GERM CELL-SPECIFIC GENE EXPRESSIONS. CELL JOURNAL (YAKHTEH)[Internet]. 2019;20(4):505-512. Available from: https://sid.ir/paper/708966/en

IEEE: Copy

MEHRDAD GHORBANLOU, ALIREZA ABDANIPOUR, REZA SHIRAZI, NASIM MALEKMOHAMMADI, SAEED SHOKRI, and REZA NEJATBAKHSH, “INDIRECT CO-CULTURE OF TESTICULAR CELLS WITH BONE MARROW MESENCHYMAL STEM CELLS LEADS TO MALE GERM CELL-SPECIFIC GENE EXPRESSIONS,” CELL JOURNAL (YAKHTEH), vol. 20, no. 4, pp. 505–512, 2019, [Online]. Available: https://sid.ir/paper/708966/en

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