ISOLATION AND CULTURE OF ADULT MICROGLIA CELLS DERIVED FROM EPILEPTIC HUMAN BRAIN TISSUE (POSTER PRESENTATION)

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GHASEMI SEPIDEH; ABDOLAHI SARA; GORJI ALI

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Journal Paper

Paper Information

Journal: Neuroscience Journal of Shefaye Khatam Year:2018 | Volume:6 | Issue:3 (SUPPLEMENT 2) Page(s): 0-0

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Title

ISOLATION AND CULTURE OF ADULT MICROGLIA CELLS DERIVED FROM EPILEPTIC HUMAN BRAIN TISSUE (POSTER PRESENTATION)

Author(s)

GHASEMI SEPIDEH | ABDOLAHI SARA | GORJI ALI | Issue Writer Certificate

Keywords

TEMPORAL LOBE EPILEPSY

EPILEPSY SURGERY

MICROGLIA

IBA1

Abstract

Introduction: TEMPORAL LOBE EPILEPSY (TLE) is the most common form of drug-resistant epilepsy in adults and generally requires surgical therapy. EPILEPSY SURGERY provides opportunities to isolate mixed glial cells, such as MICROGLIA. MICROGLIA is the resident immune cells in the brain, although the role of these cells in epilepsy remains largely undiscovered. Isolation and characterization of human MICROGLIA from epileptic tissue may improve our understanding of their basic function.Materials and Methods: Following surgical resection, human brain biopsy tissue was dissociated by mechanical and chemical digestion. Cell pellets were resuspended and cultured to medium containing growth factors and supplements. After 24 hours, floating and weakly attached cells were removed and MICROGLIA culture media was added to the plate for one week. Finally, to characterize the isolated cells, immunostaining was performed. After confirming cell phenotype, immunocytochemistry was done.Results: Our procedure obtains MICROGLIA cultures of high yield from epileptic human brain tissue using a simple method. Isolated MICROGLIA express IBA1 marker.Conclusion: MICROGLIA obtained from TLE surgery can be used for in vitro and in vivo investigation.

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APA: Copy

GHASEMI, SEPIDEH, ABDOLAHI, SARA, & GORJI, ALI. (2018). ISOLATION AND CULTURE OF ADULT MICROGLIA CELLS DERIVED FROM EPILEPTIC HUMAN BRAIN TISSUE (POSTER PRESENTATION). NEUROSCIENCE JOURNAL OF SHEFAYE KHATAM, 6(3 (SUPPLEMENT 2)), 0-0. SID. https://sid.ir/paper/709211/en

Vancouver: Copy

GHASEMI SEPIDEH, ABDOLAHI SARA, GORJI ALI. ISOLATION AND CULTURE OF ADULT MICROGLIA CELLS DERIVED FROM EPILEPTIC HUMAN BRAIN TISSUE (POSTER PRESENTATION). NEUROSCIENCE JOURNAL OF SHEFAYE KHATAM[Internet]. 2018;6(3 (SUPPLEMENT 2)):0-0. Available from: https://sid.ir/paper/709211/en

IEEE: Copy

SEPIDEH GHASEMI, SARA ABDOLAHI, and ALI GORJI, “ISOLATION AND CULTURE OF ADULT MICROGLIA CELLS DERIVED FROM EPILEPTIC HUMAN BRAIN TISSUE (POSTER PRESENTATION),” NEUROSCIENCE JOURNAL OF SHEFAYE KHATAM, vol. 6, no. 3 (SUPPLEMENT 2), pp. 0–0, 2018, [Online]. Available: https://sid.ir/paper/709211/en

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