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Information Journal Paper

Title

CONDITIONED MEDIA DERIVED FROM HUMAN ADIPOSE TISSUE MESENCHYMAL STROMAL CELLS IMPROVES PRIMARY HEPATOCYTE MAINTENANCE

Pages

  377-387

Abstract

 Objective: Recent advances in cell therapy have encouraged researchers to provide an alternative for treatment and restoration of damaged liver through using hepatocytes. However, these cells quickly lose their functional capabilitiesin vitro. Here, we aim to use the secretome of MESENCHYMAL STROMAL CELLs (MSCs) to improvein vitro maintenance conditions for hepatocytes.Materials and Methods: In this experimental study, following serum deprivation, human adipose tissue-derived MSCs (hAT-MSCs) were cultured for 24 hours under normoxic (N) and hypoxic (H) conditions. Their conditioned media (CM) were subsequently collected and labeled as N-CM (normoxia) and H-CM (hypoxia). Murine hepatocytes were isolated by perfusion of mouse liver with collagenase, and were cultured in hepatocyte basal (William’s) medium supplemented with 4% N-CM or H-CM. Untreated William’s and hepatocyte-specific media (HepZYM) were used as controls. Finally, we evaluated the survival and proliferation rates, as well as functionality and hepatocyte-specific gene expressions of the cells.Results: We observed a significant increase in viability of hepatocytes in the presence of N-CM and H-CM compared to HepZYM on day 5, as indicated by MTS (3- (4, 5-dimethylthiazol-2-yl) -5- (3-carboxymethoxyphenyl) -2- (4-sulfophenyl) -2H-tetrazolium) assay. Indocyanine green (ICG) uptake of hepatocytes in the H-CM and HepZYM groups on days 3 and 5 also suggested that H-CM maintained the hepatocytes at about the same level as the hepatocyte-specific medium.The HepZYM group had significantly higher levels of albumin (Alb) and urea secretion compared to the other groups (P<0.0001). However, there were no significant differences in cytochrome activity and cytochrome gene expression profiles among these groups. Finally, we found a slightly, but not significantly higher concentration of vascular endothelial growth factor (VEGF) in the H-CM group compared to the N-CM group (P=0.063).Conclusion: The enrichment of William’s basal medium with 4% hAT-MSC-H-CM improved some physiologic parameters in a PRIMARY HEPATOCYTE culture.

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    APA: Copy

    AZHDARI TAFTI, ZAHRA, MAHMOODI, MEHDI, HAJIZADEH, MOHAMAD REZA, EZZATIZADEH, VAHID, BAHARVAND, HOSSEIN, VOSOUGH, MASSOUD, & PIRYAEI, ABBAS. (2018). CONDITIONED MEDIA DERIVED FROM HUMAN ADIPOSE TISSUE MESENCHYMAL STROMAL CELLS IMPROVES PRIMARY HEPATOCYTE MAINTENANCE. CELL JOURNAL (YAKHTEH), 20(3), 377-387. SID. https://sid.ir/paper/712230/en

    Vancouver: Copy

    AZHDARI TAFTI ZAHRA, MAHMOODI MEHDI, HAJIZADEH MOHAMAD REZA, EZZATIZADEH VAHID, BAHARVAND HOSSEIN, VOSOUGH MASSOUD, PIRYAEI ABBAS. CONDITIONED MEDIA DERIVED FROM HUMAN ADIPOSE TISSUE MESENCHYMAL STROMAL CELLS IMPROVES PRIMARY HEPATOCYTE MAINTENANCE. CELL JOURNAL (YAKHTEH)[Internet]. 2018;20(3):377-387. Available from: https://sid.ir/paper/712230/en

    IEEE: Copy

    ZAHRA AZHDARI TAFTI, MEHDI MAHMOODI, MOHAMAD REZA HAJIZADEH, VAHID EZZATIZADEH, HOSSEIN BAHARVAND, MASSOUD VOSOUGH, and ABBAS PIRYAEI, “CONDITIONED MEDIA DERIVED FROM HUMAN ADIPOSE TISSUE MESENCHYMAL STROMAL CELLS IMPROVES PRIMARY HEPATOCYTE MAINTENANCE,” CELL JOURNAL (YAKHTEH), vol. 20, no. 3, pp. 377–387, 2018, [Online]. Available: https://sid.ir/paper/712230/en

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