Anti-biofilm Activity of Synthesized Silver Nanoparticles Using Asphodelus dendroides Extract against Antibiotic Resistant and Biofilm Forming Klebsiella pneumoniae Clinical Strains: A Laboratory Study

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SABAHI M.; HAMDI S.M.M.; MIRZAIE A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Rafsanjan University of Medical Sciences Year:2020 | Volume:18 | Issue:12 Page(s): 1233-1252

Download Full-Text

Persian Verion

View:

642

Download:

Cites:

Information Journal Paper

Title

Anti-biofilm Activity of Synthesized Silver Nanoparticles Using Asphodelus dendroides Extract against Antibiotic Resistant and Biofilm Forming Klebsiella pneumoniae Clinical Strains: A Laboratory Study

Author(s)

SABAHI M. | HAMDI S.M.M. | MIRZAIE A. | Issue Writer Certificate

Keywords

Silver nanoparticleQ1

Klebsiella pneumoniaeQ1

Antibacterial activityQ3

Anti-biofilm activityQ1

mrkA geneQ1

Abstract

Background and Objectives: Synthesis of Silver nanoparticles (AgNPs) using plant extract is cost effective, while, one of the applications of AgNPs is its antimicrobial and anti-biofilm activities. The aim of this study was to investigate the Anti-biofilm activity of synthesized AgNPs using A. dendroides against antibiotic resistant and biofilm forming clinical isolates of Klebsiella pneumoniae. Materials and Methods: In this laboratory study, the AgNPs were first synthesized using Asphodelus dendroides extract and their size and structural characteristics were determined using various physico-chemical methods. Subsequently, the antimicrobial and anti-biofilm activities of synthesized AgNPs on antibiotic resistant and biofilm forming clinical isolates of K. pneumoniae were investigated via minimum inhibitory concentration (MIC) and Real Time PCR, respectively. Results: The results of the UV-vis test showed that the synthesized AgNPs had a maximum absorbance at 420 nm. The SEM (scanning electron microscope), TEM (transmission electron microscopy) and XRD (X-ray powder diffraction) results showed that the synthesized AgNPs had a spherical structure with a mean size of 30. 41± 22 nm. Also, AgNPs had significant antimicrobial and anti-biofilm activities against K. pneumoniae strains, so that the AgNPs down-regulated the expression of biofilm coding gene (mrkA) in K. pneumonia strains (p<0. 001). Conclusion: The results of this study showed the antimicrobial and anti-biofilm activities of AgNPs against K. pneumoniae strains. According to the results, with further studies, the synthesized AgNPs may be used as biofilm inhibitors in future.

Cites

No record.

References

Cite

APA: Copy

SABAHI, M., HAMDI, S.M.M., & MIRZAIE, A.. (2020). Anti-biofilm Activity of Synthesized Silver Nanoparticles Using Asphodelus dendroides Extract against Antibiotic Resistant and Biofilm Forming Klebsiella pneumoniae Clinical Strains: A Laboratory Study. JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES, 18(12 ), 1233-1252. SID. https://sid.ir/paper/71263/en

Vancouver: Copy

SABAHI M., HAMDI S.M.M., MIRZAIE A.. Anti-biofilm Activity of Synthesized Silver Nanoparticles Using Asphodelus dendroides Extract against Antibiotic Resistant and Biofilm Forming Klebsiella pneumoniae Clinical Strains: A Laboratory Study. JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES[Internet]. 2020;18(12 ):1233-1252. Available from: https://sid.ir/paper/71263/en

IEEE: Copy

M. SABAHI, S.M.M. HAMDI, and A. MIRZAIE, “Anti-biofilm Activity of Synthesized Silver Nanoparticles Using Asphodelus dendroides Extract against Antibiotic Resistant and Biofilm Forming Klebsiella pneumoniae Clinical Strains: A Laboratory Study,” JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES, vol. 18, no. 12 , pp. 1233–1252, 2020, [Online]. Available: https://sid.ir/paper/71263/en

Related Journal Papers