EVALUATION OF ELISA METHOD USING THE EXCRETED / SECRETED ANTIGENS OF TOXOPLASMA FOR TOXOPLASMOSIS SERODIAGNOSIS IN RAT

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ABDOLLAHI S.H.; MAHMOUDZADEH POURNAKI ABAS; BAHADORAN M.

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Journal: Journal of Rafsanjan University of Medical Sciences Year:2004 | Volume:3 | Issue:4 Page(s): 232-242

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Title

EVALUATION OF ELISA METHOD USING THE EXCRETED / SECRETED ANTIGENS OF TOXOPLASMA FOR TOXOPLASMOSIS SERODIAGNOSIS IN RAT

Author(s)

ABDOLLAHI S.H. | MAHMOUDZADEH POURNAKI ABAS | BAHADORAN M. | Issue Writer Certificate

Keywords

TOXOPLASMOSISQ3

EXCRETED/SECRETED ANTIGENSQ2

ELISAQ4

TOXOPLASMA GONDIIQ2

Abstract

Background: Many studies have reported that TOXOPLASMA GONDII EXCRETED/SECRETED ANTIGENS (E/SA) appear to be a suitable marker for TOXOPLASMOSIS serodiagnosis. Most of those studies have used E/SA obtained from supernatant of toxoplasma cell culture, or by incubating tachyzoites in cell free media (RPMI- 1640). The present study, evaluated the ELISA method, using the components of peritoneal fluid of infected mice (as another source of E/SA), for TOXOPLASMOSIS diagnosis in rat. Materials and Methods: Peritoneal fluids of infected mice by interaperitoneal inoculation (IP) with toxoplasma tachyzoits were collected after 3 days of infection and centrifuged at 750×g for 15 min, then the supernatant was precipitated with ammonium soleplates solution (30% saturated) Forty noninfected (male) rats (7-10 weeks old) were injected with 4×106 toxoplasma tachyzoits IP and their serum samples were collected at 8, 15, 22 and 60 days after the infection. Then 10 samples from each day were tested by Dye- test. The sera of 15 and 60 days after infection of all animals were selected as suitable samples. Then the sera of these days were tested by the dye-test and ELISA using E/SA.Results: The cut-off point of ELISA with 99% confidence was found to be 0.33 and optical density (OD) of all the sera samples of 15 and 60 days after infection and 2 negative sera were over than test cut-off. Moreover, sensitivity and specificity of the method were determined to be 100% and 95%, respectively.Conclusion: Our findings showed that ELISA in the condition of using ammonium sulphate precipitation has a good enough sensitivity and specificity for TOXOPLASMOSIS diagnosis in rat.

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References

EVALUATION OF DOT-ELLSA METHOD USING E/SA FOR THE DIAGNOSIS OF TOXOPLASMOSIS IN RAT

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APA: Copy

ABDOLLAHI, S.H., MAHMOUDZADEH POURNAKI, ABAS, & BAHADORAN, M.. (2004). EVALUATION OF ELISA METHOD USING THE EXCRETED / SECRETED ANTIGENS OF TOXOPLASMA FOR TOXOPLASMOSIS SERODIAGNOSIS IN RAT. JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES, 3(4), 232-242. SID. https://sid.ir/paper/71403/en

Vancouver: Copy

ABDOLLAHI S.H., MAHMOUDZADEH POURNAKI ABAS, BAHADORAN M.. EVALUATION OF ELISA METHOD USING THE EXCRETED / SECRETED ANTIGENS OF TOXOPLASMA FOR TOXOPLASMOSIS SERODIAGNOSIS IN RAT. JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES[Internet]. 2004;3(4):232-242. Available from: https://sid.ir/paper/71403/en

IEEE: Copy

S.H. ABDOLLAHI, ABAS MAHMOUDZADEH POURNAKI, and M. BAHADORAN, “EVALUATION OF ELISA METHOD USING THE EXCRETED / SECRETED ANTIGENS OF TOXOPLASMA FOR TOXOPLASMOSIS SERODIAGNOSIS IN RAT,” JOURNAL OF RAFSANJAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES, vol. 3, no. 4, pp. 232–242, 2004, [Online]. Available: https://sid.ir/paper/71403/en

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