INHIBITION OF BREAST CANCER METASTASIS BY CO-TRANSFECTION OF MIR-31/193B-MIMICS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HASHEMI ZAHRA SADAT; FOROUZANDEH MOGHADAM MEHDI; FAROKHIMANESH SAMILA; RAJABIBAZL MASOUMEH; SADRODDINY ESMAEIL

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Basic Medical Sciences Year:2018 | Volume:21 | Issue:4 Page(s): 427-433

Download Full-Text

View:

282

Download:

273

Cites:

Information Journal Paper

Title

INHIBITION OF BREAST CANCER METASTASIS BY CO-TRANSFECTION OF MIR-31/193B-MIMICS

Author(s)

Keywords

BREAST CANCER

METASTASIS

MIR-31

MIR-193B

RHOA

UPA

Abstract

Objective (s): Various studies have been conducted to reduce the metastatic behavior of cancerous cells. In this regard, ectopic expression of anti-metastatic microRNAs by miR-mimic and miR-restoration based therapies could bring new insights to the field. In the present study, the consequences of co-transfecting BREAST CANCER cell lines with MIR-193B and MIR-31 were investigated via invasion and migration assays.Materials and Methods: Double stranded oligonucleotide of mature MIR-193B-3p and MIR-31-5p were cloned into pcDNA 6.2gw/EmGFP plasmid. The resulting plasmids were used for transfection. Real time- PCR was performed to assess the expression of MIR-193B and MIR-31 as well as Ras homolog gene family member A (RhoA) and urokinase-type plasminogen activator (uPA) as miR targets. Scratch, Transwell migration and Matrigel invasion assays were carried out to assess the extent of migration and invasion of cell lines.Results: The most significant increase in expression of miRs belonged to the single transfection of mimic-miRs in MDA-MB231. Although the co-transfection was not as successful as single transfection in miR expression, it was significantly more effective in inhibition of the cells invasive potential.Conclusion: Although the miR-restoration therapy based on co-transfection of two miRs could be less effective in expression of each miRNA, the resulting decrease in metastatic behavior of the cells is more significant due to collective effect of co-transfection to decrease target gene expression. Our results revealed that employing this sort of combinatorial strategies could lead to more efficient reduction in metastatic behavior. It seems that using this strategy would bring about more successful therapeutic outcomes.

Cites

No record.

References

No record.

Cite

APA: Copy

HASHEMI, ZAHRA SADAT, FOROUZANDEH MOGHADAM, MEHDI, FAROKHIMANESH, SAMILA, RAJABIBAZL, MASOUMEH, & SADRODDINY, ESMAEIL. (2018). INHIBITION OF BREAST CANCER METASTASIS BY CO-TRANSFECTION OF MIR-31/193B-MIMICS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 21(4), 427-433. SID. https://sid.ir/paper/714301/en

Vancouver: Copy

HASHEMI ZAHRA SADAT, FOROUZANDEH MOGHADAM MEHDI, FAROKHIMANESH SAMILA, RAJABIBAZL MASOUMEH, SADRODDINY ESMAEIL. INHIBITION OF BREAST CANCER METASTASIS BY CO-TRANSFECTION OF MIR-31/193B-MIMICS. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2018;21(4):427-433. Available from: https://sid.ir/paper/714301/en

IEEE: Copy

ZAHRA SADAT HASHEMI, MEHDI FOROUZANDEH MOGHADAM, SAMILA FAROKHIMANESH, MASOUMEH RAJABIBAZL, and ESMAEIL SADRODDINY, “INHIBITION OF BREAST CANCER METASTASIS BY CO-TRANSFECTION OF MIR-31/193B-MIMICS,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 21, no. 4, pp. 427–433, 2018, [Online]. Available: https://sid.ir/paper/714301/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops