Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

316
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

227
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

MORPHOLOGICAL, ULTRASTRUCTURAL, AND MOLECULAR ASPECTS OF IN VITRO MOUSE EMBRYO IMPLANTATION ON HUMAN ENDOMETRIAL MESENCHYMAL STROMAL CELLS IN THE PRESENCE OF STEROID HORMONES AS AN IMPLANTATION MODEL

Pages

  369-376

Abstract

 Objective: This experimental study aimed to evaluate the effects of 17b-estradiol (E2) and PROGESTERONE (P4) on the interaction between mouse embryo and human endometrial MESENCHYMAL STROMAL CELLS, and gene expressions related to IMPLANTATION [aV and b3 integrins, INTERLEUKIN-1 RECEPTOR (IL-1R), and leukemia inhibitory factor receptor (LIFR)] using an in vitro twodimensional model.Materials and Methods: In this experimental study, the endometrial stromal cells were isolated enzymatically and mechanically, and cultured to the fourth passage. Next, their immunophenotype was confirmed by flow cytometric analysis as MESENCHYMAL STROMAL CELLS. The cells were cultured as either the experimental group in the presence of E2 (0.3 nmol) and P4 (63.5 nmol) or control group without any hormone treatment. Mouse blastocysts were co-cultured with endometrial MESENCHYMAL STROMAL CELLS in both groups for 48 hours. Their interaction was assessed under an inverted microscope and scanning electron microscopy (SEM). Expressions ofαV and β3 integrins, LIFR, and IL-1R genes were analyzed by real-time reverse transcription-polymerase chain reaction (RT-PCR).Results: Similar observations were seen in both groups by light microscopy and SEM. We observed the presence of pinopode-like structures and cell secretions on the apical surfaces of endometrial MESENCHYMAL STROMAL CELLS in both groups. The trophoblastic cells expanded and interacted with the mesenchymal monolayer cells. At the molecular level, expression ofIL-1R significantly increased in the hormonal treated group compared to the control (P≤0.05). Expressions of the other genes did not differ.Conclusion: This study has shown that co-culture of endometrial MESENCHYMAL STROMAL CELLS with mouse embryo in media that contained E2 (0.3 nmol) and P4 (63.5 nmol) could effectively increase the expression ofIL-1R, which is involved in embryo IMPLANTATION. However, there were no significant effects on expressions ofαV and b3 integrins, LIFR, and on the morphology and ultrastructure of endometrial MESENCHYMAL STROMAL CELLS.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    RAHIMIPOUR, MARZIEH, SALEHNIA, MOJDEH, & JAFARABADI, MINA. (2018). MORPHOLOGICAL, ULTRASTRUCTURAL, AND MOLECULAR ASPECTS OF IN VITRO MOUSE EMBRYO IMPLANTATION ON HUMAN ENDOMETRIAL MESENCHYMAL STROMAL CELLS IN THE PRESENCE OF STEROID HORMONES AS AN IMPLANTATION MODEL. CELL JOURNAL (YAKHTEH), 20(3), 369-376. SID. https://sid.ir/paper/715441/en

    Vancouver: Copy

    RAHIMIPOUR MARZIEH, SALEHNIA MOJDEH, JAFARABADI MINA. MORPHOLOGICAL, ULTRASTRUCTURAL, AND MOLECULAR ASPECTS OF IN VITRO MOUSE EMBRYO IMPLANTATION ON HUMAN ENDOMETRIAL MESENCHYMAL STROMAL CELLS IN THE PRESENCE OF STEROID HORMONES AS AN IMPLANTATION MODEL. CELL JOURNAL (YAKHTEH)[Internet]. 2018;20(3):369-376. Available from: https://sid.ir/paper/715441/en

    IEEE: Copy

    MARZIEH RAHIMIPOUR, MOJDEH SALEHNIA, and MINA JAFARABADI, “MORPHOLOGICAL, ULTRASTRUCTURAL, AND MOLECULAR ASPECTS OF IN VITRO MOUSE EMBRYO IMPLANTATION ON HUMAN ENDOMETRIAL MESENCHYMAL STROMAL CELLS IN THE PRESENCE OF STEROID HORMONES AS AN IMPLANTATION MODEL,” CELL JOURNAL (YAKHTEH), vol. 20, no. 3, pp. 369–376, 2018, [Online]. Available: https://sid.ir/paper/715441/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top