Evaluation of Different Phenotypic and Genotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus (MRSA)

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Koupahi Hossein; Honarmand Jahromi Sahar; RAHBAR MOHAMMAD

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Journal: IRANIAN JOURNAL OF PATHOLOGY (IJP) Year:2016 | Volume:11 | Issue:4 Page(s): 370-376

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Title

Evaluation of Different Phenotypic and Genotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus (MRSA)

Author(s)

Koupahi Hossein | Honarmand Jahromi Sahar | RAHBAR MOHAMMAD | Issue Writer Certificate

Keywords

Staphylococcus aureus mecA gene CHOROM Agar MRSA Oxacillin screen Agar Methicillin Resistant

Abstract

Background: Methicillin resistant Staphylococcus aureus (MRSA) has been emerged as a nosocomial and community acquired pathogen worldwide. There are many challenges for laboratory detection of MRSA. The aim of this study was to compare different phenotypic methods with PCR based method as a gold standard for detection of mecA gene. Methods: A total of 220 clinical isolates of S. aureus which were isolated from various clinical specimens from September 2013 until the June of 2014 in Milad Hospital of Tehran, Iran was subject of our study. Methicillin resistance was determined by oxacillin and cefoxitin disks, oxacillin screen agar and CHROMagar™ MRSA medium. The results of these methods were compared with mecA gene based PCR method as a gold standard method. Results: Among 220 isolates from S. aureus, 105 (47. 72%) isolates were positive for mecA gene by PCR method. The results of cefoxitin disk diffusion method with 100% sensitivity and specificity was the same as PCR method. CHROMagar™ MRSA medium had 98. 13% sensitivity and 100% specificity. Oxacillin disk diffusion and oxacillin screen agar had 95. 42% and 97. 22% sensitivity respectively. Conclusion: Result of cefoxitin disk diffusion method with 100% sensitivity and specificity was the same as PCR method for detection mecA gene. Cefoxitin disk diffusion method can be used as an alternative method of PCR for detection of MRSA.

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APA: Copy

Koupahi, Hossein, Honarmand Jahromi, Sahar, & RAHBAR, MOHAMMAD. (2016). Evaluation of Different Phenotypic and Genotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus (MRSA). IRANIAN JOURNAL OF PATHOLOGY (IJP), 11(4), 370-376. SID. https://sid.ir/paper/715655/en

Vancouver: Copy

Koupahi Hossein, Honarmand Jahromi Sahar, RAHBAR MOHAMMAD. Evaluation of Different Phenotypic and Genotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus (MRSA). IRANIAN JOURNAL OF PATHOLOGY (IJP)[Internet]. 2016;11(4):370-376. Available from: https://sid.ir/paper/715655/en

IEEE: Copy

Hossein Koupahi, Sahar Honarmand Jahromi, and MOHAMMAD RAHBAR, “Evaluation of Different Phenotypic and Genotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus (MRSA),” IRANIAN JOURNAL OF PATHOLOGY (IJP), vol. 11, no. 4, pp. 370–376, 2016, [Online]. Available: https://sid.ir/paper/715655/en

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