Diagnostic Yield of a Direct Quantitative Smear of Lower Respiratory Tract Secretions in Patients with Suspected Pneumonia Compared to a Semi-quantitative Culture

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SHOKOUHI SHERVIN; DARAZAM ILAD ALAVI; SADEGHI MARYAM; GACHKAR LATIF; DOLATSHAHI SAMANEH

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Journal: TANAFFOS Year:2017 | Volume:16 | Issue:1 Page(s): 9-12

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Title

Diagnostic Yield of a Direct Quantitative Smear of Lower Respiratory Tract Secretions in Patients with Suspected Pneumonia Compared to a Semi-quantitative Culture

Author(s)

Keywords

Semi-quantitative culture

Bacteria

Pneumonia

Bronchoalveolar lavage

Intensive care units

Abstract

Background: Microorganism isolation from respiratory tract specimens is the standard of care in patients with suspected nosocomial and ventilator associated Pneumonia. However, these methods are time-consuming and are influenced by several factors. A direct quantitative smear (DQS) with proper staining may be an easy, cost-effective, rapid method. We evaluated the diagnostic yield of direct smears compared to Semi-quantitative culture methods. Materials and Methods: Hospitalized, intubated patients with clinically suspected Pneumonia and patients who underwent diagnostic bronchoscopic alveolar lavage (BAL) and trans-endotracheal aspiration (TEA) were enrolled in a prospective study. The obtained specimens were Gram stained and microorganisms were computed per 10 high-power fields (HPFs) of light microscopy. All samples were cultured by a standard semi-quantitative method. Colony-forming units (CFU) >104/mL and >105 CFU/mL were reported as culture-positive for BAL and TEA, respectively. Results: A total of 331 respiratory specimens were analyzed. Based on culture results, the best cut-off point was 35 microorganisms in 10 HPFs of microscopy and provided 90. 4% sensitivity and 90. 8% specificity. The best cut-off point for 25 microorganisms in 10 fields of light microscopy provided 95. 2% sensitivity and 85. 7% specificity. Conclusion: A DQS obtained by BAL and TEA may be a reliable and rapid method to diagnose Pneumonia and anticipate Semi-quantitative culture results. The sensitivity and specificity of a direct smear have adequate diagnostic yield to recommend it as an adjunct to microorganism-isolation methods.

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APA: Copy

SHOKOUHI, SHERVIN, DARAZAM, ILAD ALAVI, SADEGHI, MARYAM, GACHKAR, LATIF, & DOLATSHAHI, SAMANEH. (2017). Diagnostic Yield of a Direct Quantitative Smear of Lower Respiratory Tract Secretions in Patients with Suspected Pneumonia Compared to a Semi-quantitative Culture. TANAFFOS, 16(1), 9-12. SID. https://sid.ir/paper/717797/en

Vancouver: Copy

SHOKOUHI SHERVIN, DARAZAM ILAD ALAVI, SADEGHI MARYAM, GACHKAR LATIF, DOLATSHAHI SAMANEH. Diagnostic Yield of a Direct Quantitative Smear of Lower Respiratory Tract Secretions in Patients with Suspected Pneumonia Compared to a Semi-quantitative Culture. TANAFFOS[Internet]. 2017;16(1):9-12. Available from: https://sid.ir/paper/717797/en

IEEE: Copy

SHERVIN SHOKOUHI, ILAD ALAVI DARAZAM, MARYAM SADEGHI, LATIF GACHKAR, and SAMANEH DOLATSHAHI, “Diagnostic Yield of a Direct Quantitative Smear of Lower Respiratory Tract Secretions in Patients with Suspected Pneumonia Compared to a Semi-quantitative Culture,” TANAFFOS, vol. 16, no. 1, pp. 9–12, 2017, [Online]. Available: https://sid.ir/paper/717797/en

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