The Synergistic Effect of Glucagon-Like Peptide-1 and Chamomile Oil on Differentiation of Mesenchymal Stem Cells into Insulin-Producing Cells

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Saghahazrati Saeid; AYATOLLAHI SEYED ABDOL MAJID; KOBARFARD FARZAD; MINAEII ZANGI MOHAMMAD BAGHER

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Journal Paper

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Journal: Cell Journal (Yakhteh) Year:2020 | Volume:21 | Issue:4 Page(s): 371-378

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Title

The Synergistic Effect of Glucagon-Like Peptide-1 and Chamomile Oil on Differentiation of Mesenchymal Stem Cells into Insulin-Producing Cells

Author(s)

Saghahazrati Saeid | AYATOLLAHI SEYED ABDOL MAJID | KOBARFARD FARZAD | MINAEII ZANGI MOHAMMAD BAGHER | Issue Writer Certificate

Keywords

Chamomile Oil

Differentiation

Glucagon-Like Peptide-1

Insulin-Producing Cells

Mesenchymal Stem Cell

Abstract

Objective: Glucagon-Like Peptide-1 (GLP-1) has attracted tremendous attention for treatment of diabetes. Likewise, it seems that active ingredients of Chamomile Oil might have anti-diabetic effects. This work was conducted to investigate the effects of the combination of GLP-1 and Chamomile Oil on Differentiation of Mesenchymal Stem Cells (MSCs) into functional Insulin-Producing Cells (IPCs). Materials and Methods: In this experimental study, adipose MSCs derived from the adult male New Zealand white rabbits were assigned into four groups: control (without any treatment); GLP-1 (in which cells were treated with 10 nM GLP-1 every other day for 5 days); Chamomile Oil (in which cells were treated with 100 ug/ml Matricaria chamomilla L. flower oil every other day for 5 days); and GLP-1+ Chamomile Oil (in which cells were treated with 10 nM GLP-1 and 100 μ g/ml M. chamomilla flower oil every other day for 5 days). Characterization of isolated MSCs was performed using flow cytometry, Alizarin red S staining and Oil red O staining. The expressions of genes specific for IPCs were measured using reverse transcriptase-polymerase chain reaction (RT-PCR) assay. Measurement of insulin and the cleaved connecting peptide (C-peptide) in response to different concentrations of glucose, were performed using ELISA kits. Results: Our results demonstrated that isolated cells highly expressed MSC markers and were able to differentiate into osteocytes and adipocytes. Additionally, using GLP-1 in combination with Chamomile Oil exhibited higher levels of IPCs gene markers including NK homeobox gene 2. 2 (NKX-2. 2), paired box gene 4 (PAX4), insulin (INS) and pancreatic duodenal homeobox-1 (PDX1) as well as insulin and C-peptide secretion in response to different glucose concentrations compared to GLP-1 or Chamomile Oil alone (P<0. 05). Conclusion: Collectively, these findings establish a substantial foundation for using peptides in combination with natural products to obtain higher efficiency in regenerative medicine and peptide therapy.

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APA: Copy

Saghahazrati, Saeid, KOBARFARD, FARZAD, & MINAEII ZANGI, MOHAMMAD BAGHER. (2020). The Synergistic Effect of Glucagon-Like Peptide-1 and Chamomile Oil on Differentiation of Mesenchymal Stem Cells into Insulin-Producing Cells. CELL JOURNAL (YAKHTEH), 21(4), 371-378. SID. https://sid.ir/paper/719634/en

Vancouver: Copy

Saghahazrati Saeid, KOBARFARD FARZAD, MINAEII ZANGI MOHAMMAD BAGHER. The Synergistic Effect of Glucagon-Like Peptide-1 and Chamomile Oil on Differentiation of Mesenchymal Stem Cells into Insulin-Producing Cells. CELL JOURNAL (YAKHTEH)[Internet]. 2020;21(4):371-378. Available from: https://sid.ir/paper/719634/en

IEEE: Copy

Saeid Saghahazrati, FARZAD KOBARFARD, and MOHAMMAD BAGHER MINAEII ZANGI, “The Synergistic Effect of Glucagon-Like Peptide-1 and Chamomile Oil on Differentiation of Mesenchymal Stem Cells into Insulin-Producing Cells,” CELL JOURNAL (YAKHTEH), vol. 21, no. 4, pp. 371–378, 2020, [Online]. Available: https://sid.ir/paper/719634/en

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