مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

204
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

113
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Protective effect of bioactive compounds from Lonicera japonica Thunb. against H2O2‐ induced cytotoxicity using neonatal rat cardiomyocytes

Pages

  97-105

Abstract

 Objective(s): Pharmacological studies showed that the extracts of Jin Yin Hua and its active constituents have lipid lowering, antipyretic, hepatoprotective, cytoprotective, antimicrobial, antibiotic, antioxidative, antiviral, and anti‐ inflammatory effects. The purpose of the present study was to investigate the protective effects of Caffeoylquinic acids (CQAs) from Jin Yin Hua against hydrogen peroxide (H2O2)‐ induced and hypoxia‐ induced cytotoxicity using neonatal rat Cardiomyocytes. Materials and Methods: Seven CQAs (C1 to C7) isolated and identified from Jin Yin Hua were used to examine the effects of H2O2‐ induced and hypoxia‐ induced cytotoxicity. We studied C4 and C6 as preventative bioactive compounds of the reactive oxygen species (ROS) production, apoptotic pathway, and apoptosis‐ related gene expression. Results: C4 and C6 were screened as bioactive compounds to exert a cytoprotective effect against oxidative injury. Pretreatment with C4 and C6, dose‐ dependently attenuated hypoxia‐ induced ROS production and reduced the ratio of GSSG/GStotal. Western blot data revealed that the inhibitory effect of C4 on H2O2‐ induced up and down‐ regulation of Bcl‐ 2, Bax, caspase‐ 3, and cleaved caspase‐ 3. Apoptosis was evaluated by detection of DNA fragmentation using TUNEL assay, and quantified with Annexin V/PI staining. Conclusion: In vitro experiments revealed that both C4 and C6 protect Cardiomyocytes from necrosis and apoptosis during H2O2‐ induced injury, via inhibiting the generation of ROS and activation of caspase‐ 3 apoptotic pathway. These results demonstrated that CQAs might be a class of compounds which possess potent myocardial protective activity against the ischemic heart diseases related to Oxidative stress.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    WANG, CHEN, WANG, GANG, LIU, HONG, & Hou, Yun long. (2016). Protective effect of bioactive compounds from Lonicera japonica Thunb. against H2O2‐ induced cytotoxicity using neonatal rat cardiomyocytes. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 19(1), 97-105. SID. https://sid.ir/paper/719650/en

    Vancouver: Copy

    WANG CHEN, WANG GANG, LIU HONG, Hou Yun long. Protective effect of bioactive compounds from Lonicera japonica Thunb. against H2O2‐ induced cytotoxicity using neonatal rat cardiomyocytes. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2016;19(1):97-105. Available from: https://sid.ir/paper/719650/en

    IEEE: Copy

    CHEN WANG, GANG WANG, HONG LIU, and Yun long Hou, “Protective effect of bioactive compounds from Lonicera japonica Thunb. against H2O2‐ induced cytotoxicity using neonatal rat cardiomyocytes,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 19, no. 1, pp. 97–105, 2016, [Online]. Available: https://sid.ir/paper/719650/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button