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Information Journal Paper

Title

The miR-383-LDHA axis regulates cell proliferation, invasion and glycolysis in hepatocellular cancer

Pages

  187-192

Abstract

 To explore the correlation between expression patterns and functions of MiR-383 and LDHA in Hepatocellular cancer (HCC). Materials and Methods: We detected the expression of MiR-383 and LDHA in 30 HCC tissues and their matched adjacent normal tissues using qRT-PCR. Then we performed MTT assay, foci formation assay, transwell migration assay, glucose uptake assay and lactate production assay to explore the function of MiR-383 in cell proliferation, invasion and glycolysis in HCC cell lines. Luciferase reporter assay was used to explore whether LDHA was a target gene of MiR-383. Western blot and qRT-PCR were used to further confirm LDHA was targeted by MiR-383. Then the above functional experiments were repeated to see whether the function of LDHA could be inhibited by MiR-383. Results: The results of qRT-PCR showed that MiR-383 was down-regulated in HCC tissues compared with their matched adjacent normal tissues. Functional experiments showed that overexpression of MiR-383 significantly suppressed cell proliferation, invasion and glycolysis. Luciferase reporter assay showed LDHA was a target gene of MiR-383 and expression of LDHA was inversely correlated with that of MiR-383 in HCC. Besides, increased cell proliferation, invasion and glycolysis triggered by LDHA could be inhibited by overexpression of MiR-383 in HCC cell lines. Conclusion: Our study proved that MiR-383 is down-regulated in HCC and acts as a tumor suppressor through targeting LDHA. Targeting the MiR-383-LDHA axis might be a promising strategy in HCC treatment.

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    APA: Copy

    Fang, Zhixiong, He, Langqiu, Jia, Hui, Huang, Qiusheng, Zhang, Zhiwei, & Chen, Dan. (2017). The miR-383-LDHA axis regulates cell proliferation, invasion and glycolysis in hepatocellular cancer. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 20(2), 187-192. SID. https://sid.ir/paper/720398/en

    Vancouver: Copy

    Fang Zhixiong, He Langqiu, Jia Hui, Huang Qiusheng, Zhang Zhiwei, Chen Dan. The miR-383-LDHA axis regulates cell proliferation, invasion and glycolysis in hepatocellular cancer. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2017;20(2):187-192. Available from: https://sid.ir/paper/720398/en

    IEEE: Copy

    Zhixiong Fang, Langqiu He, Hui Jia, Qiusheng Huang, Zhiwei Zhang, and Dan Chen, “The miR-383-LDHA axis regulates cell proliferation, invasion and glycolysis in hepatocellular cancer,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 20, no. 2, pp. 187–192, 2017, [Online]. Available: https://sid.ir/paper/720398/en

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