The effect of amniotic membrane extract on umbilical cord blood mesenchymal stem cell expansion: is there any need to save the amniotic membrane besides the umbilical cord blood?

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VOJDANI ZAHRA; BABAEI ALI; VASAGHI ATTIYEH; HABIBAGAHI MOJTABA; TALAEI KHOZANI TAHEREH

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Journal Paper

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Journal: Iranian Journal of Basic Medical Sciences Year:2016 | Volume:19 | Issue:1 Page(s): 89-96

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Title

The effect of amniotic membrane extract on umbilical cord blood mesenchymal stem cell expansion: is there any need to save the amniotic membrane besides the umbilical cord blood?

Author(s)

Keywords

Amnion

Basic-fibroblast growth factor

Wharton’

s jelly

Mesenchymal stem cell

Abstract

Objective(s): Umbilical cord blood is a good source of the Mesenchymal stem cells that can be banked, expanded and used in regenerative medicine. The objective of this study was to test whether amniotic membrane extract, as a rich source of growth factors such as Basic-fibroblast growth factor, can promote the proliferation potential of the umbilical cord Mesenchymal stem cells. Materials and Methods: The study design was interventional. Umbilical cord Mesenchymal stem cells were isolated from voluntary healthy infants from hospitals in Shiraz, Iran, cultured in the presence of Basic-fibroblast growth factor and amniotic membrane extracts (from pooled-samples), and compared with control cultures. Proliferation assay was performed and duplication number and time were calculated. The expression of stem cell’ s specific markers and the differentiation capacity toward osteogenic and adipogenic lineages were evaluated. Results: Amniotic membrane extract led to a significant increase in the proliferation rate and duplication number and a decrease in the duplication time without any change in the cell morphology. Both amniotic membrane extract and Basic-fibroblast growth factor altered the expressing of CD44 and CD105 in cell population. Treating Basic-fibroblast growth factor but not the amniotic membrane extract favored the differentiation potential of the stem cells toward osteogenic lineage. Conclusion: The amniotic membrane extract administration accelerated cell proliferation and modified the CD marker characteristics which may be due to the induction of differentiation toward a specific lineage. Amniotic membrane extract may enhance the proliferation rate and duplication number of the stem cell through changing the duplication time.

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APA: Copy

VOJDANI, ZAHRA, BABAEI, ALI, VASAGHI, ATTIYEH, HABIBAGAHI, MOJTABA, & TALAEI KHOZANI, TAHEREH. (2016). The effect of amniotic membrane extract on umbilical cord blood mesenchymal stem cell expansion: is there any need to save the amniotic membrane besides the umbilical cord blood?. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 19(1), 89-96. SID. https://sid.ir/paper/720654/en

Vancouver: Copy

VOJDANI ZAHRA, BABAEI ALI, VASAGHI ATTIYEH, HABIBAGAHI MOJTABA, TALAEI KHOZANI TAHEREH. The effect of amniotic membrane extract on umbilical cord blood mesenchymal stem cell expansion: is there any need to save the amniotic membrane besides the umbilical cord blood?. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2016;19(1):89-96. Available from: https://sid.ir/paper/720654/en

IEEE: Copy

ZAHRA VOJDANI, ALI BABAEI, ATTIYEH VASAGHI, MOJTABA HABIBAGAHI, and TAHEREH TALAEI KHOZANI, “The effect of amniotic membrane extract on umbilical cord blood mesenchymal stem cell expansion: is there any need to save the amniotic membrane besides the umbilical cord blood?,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 19, no. 1, pp. 89–96, 2016, [Online]. Available: https://sid.ir/paper/720654/en

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