مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

261
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

179
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

1

Information Journal Paper

Title

Gestational diabetes leads to down-regulation of CDK4-pRB-E2F1 pathway genes in pancreatic islets of rat offspring

Pages

  150-154

Abstract

 The link between a hyperglycemic intrauterine environment and the development of diabetes later in life has been observed in Offspring exposed to gestational diabetes mellitus (GDM), but the underlying mechanisms for this phenomenon are still not clear. Reduced β-cells mass is a determinant in the development of diabetes (type 1 and type 2 diabetes). Some recent studies have provided evidence that the CDK4-pRB-E2F1 regulatory pathway is involved in β-cells proliferation. Therefore, we postulated that GDM exposure impacts the Offspring’ s β-cells by disruption in the CDK4-pRB-E2F1 pathway. Materials and Methods: Adult Wistar rats were randomly allocated in control and diabetic group. The experimental group received 40 mg/kg/body weight of streptozotocin (STZ) on day zero of gestation. After delivery, diabetic Offspring of GDM mothers and control dams at the age of 15 week were randomly scarified and pancreases were harvested. Langerhans islets of diabetic and control groups were digested by collagenase digestion technique. After RNA extraction, we investigated the expressions of the kir 6. 2 and CDK4-pRB-E2F1 pathway genes by quantitative real-time PCR. Results: GDM reduced the expression of CDK4-pRB-E2F1 pathway genes in Langerhans islets cells of Offspring. CDK4, pRB and E2F1 pathway genes were downregulated in diabetic islets by 51%, 35% and 84%, respectively. Also, the expression of Kir 6. 2 was significantly decreased in diabetic islets by 88%. Conclusion: We suggest that the effect of gestational diabetes on Offspring’ s β-cells may be primarily caused by the suppression of CDK4-pRB-E2F1 pathway.

Cites

References

  • No record.

    • Cite

    APA: Copy

    NAZARI, ZAHRA, NABIUNI, MOHAMMAD, SAEIDI, MOHSEN, & GOLALIPOUR, MOHAMMAD JAFAR. (2017). Gestational diabetes leads to down-regulation of CDK4-pRB-E2F1 pathway genes in pancreatic islets of rat offspring. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 20(2), 150-154. SID. https://sid.ir/paper/720701/en

    Vancouver: Copy

    NAZARI ZAHRA, NABIUNI MOHAMMAD, SAEIDI MOHSEN, GOLALIPOUR MOHAMMAD JAFAR. Gestational diabetes leads to down-regulation of CDK4-pRB-E2F1 pathway genes in pancreatic islets of rat offspring. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2017;20(2):150-154. Available from: https://sid.ir/paper/720701/en

    IEEE: Copy

    ZAHRA NAZARI, MOHAMMAD NABIUNI, MOHSEN SAEIDI, and MOHAMMAD JAFAR GOLALIPOUR, “Gestational diabetes leads to down-regulation of CDK4-pRB-E2F1 pathway genes in pancreatic islets of rat offspring,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 20, no. 2, pp. 150–154, 2017, [Online]. Available: https://sid.ir/paper/720701/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button