مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

194
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

116
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

MiR-570 inhibits cell proliferation and glucose metabolism by targeting IRS1 and IRS2 in human chronic myelogenous leukemia

Pages

  481-488

Abstract

 Objective(s): Chronic Myelogenous Leukemia (CML) is a chronic myeloproliferative disorder characterized by the accumulation of myeloid cells with a chromosomal translocation known as the Philadelphia chromosome. In this study, we investigated the roles of miR-570 in CML development. Materials and Methods: Expression of miR-570 in CML samples and cell lines was determined by qRT-PCR. Glucose uptake and ATP concentration detection assays were used to analyze cell glucose metabolism. MTT and western blot assays were performed for cell proliferation and apoptosis, respectively. The targets of miR-570 were predicted by bioinformatics and confirmed using luciferase activity, qRT-PCR and western blot assays. Results: The expression levels of miR-570 were significantly reduced in CML clinical samples and cells. Overexpression of miR-570 inhibited cell proliferation, promoted apoptosis, and suppressed glucose metabolism in CML cells. Insulin receptor substrates (IRS) 1 and IRS2 were identified as direct targets of miR-570. IRS1 or IRS2 were knocked down in K562 cells. Loss of IRS1/2 expression led to suppressed cell proliferation, elevated apoptosis, and decreased glucose metabolism in CML cells, which is consistent with their roles as miR-570 targets. Conclusion: miR-570 directly targeted IRS1 and IRS2 in CML, suppressing cell proliferation and glucose metabolism. miR-570 may provide a strategy for CML therapy.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    Zhao, Hong, LIU, FEI, Jia, Ruichun, Chang, Huiying, Li, Haixia, Miao, Meijuan, WANG, HUI, & Yang, Zhiping. (2017). MiR-570 inhibits cell proliferation and glucose metabolism by targeting IRS1 and IRS2 in human chronic myelogenous leukemia. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 20(5), 481-488. SID. https://sid.ir/paper/720744/en

    Vancouver: Copy

    Zhao Hong, LIU FEI, Jia Ruichun, Chang Huiying, Li Haixia, Miao Meijuan, WANG HUI, Yang Zhiping. MiR-570 inhibits cell proliferation and glucose metabolism by targeting IRS1 and IRS2 in human chronic myelogenous leukemia. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2017;20(5):481-488. Available from: https://sid.ir/paper/720744/en

    IEEE: Copy

    Hong Zhao, FEI LIU, Ruichun Jia, Huiying Chang, Haixia Li, Meijuan Miao, HUI WANG, and Zhiping Yang, “MiR-570 inhibits cell proliferation and glucose metabolism by targeting IRS1 and IRS2 in human chronic myelogenous leukemia,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 20, no. 5, pp. 481–488, 2017, [Online]. Available: https://sid.ir/paper/720744/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button