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Information Journal Paper

Title

Ameliorating effect of encapsulated hepatocyte-like cells derived from umbilical cord in high mannuronic alginate scaffolds on acute liver failure in rats

Pages

  928-935

Abstract

 Objective(s): In this study, effects of encapsulated umbilical cord stem cells (UCSCs)-derived hepatocyte-like cells (HLCs) in high mannuronic alginate scaffolds was investigated on CCl4-induced acute liver failure (ALF) in rats. Material and Methods: UCSCs were encapsulated in high mannuronic alginate scaffolds. Then the UCSCs differentiated into HLCs for treatment of CCl4-induced ALF in rats. Thirty rats randomly divided into 5 groups: Intoxicated group received only CCl4 to induce ALF. In other groups including cellfree, UCSCs and HLCs, alginate scaffolds were transplanted into the liver 4 days after CCl4 injection. Biochemical markers including albumin (ALB), blood urea nitrogen (BUN), alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were evaluated. Histological changes and gene expression of ALB, alpha-fetoprotein (AFP), and cytokeratin 18 (CK-18) were also assessed. Results: Expression of CK-18 significantly increased in HLCs compared to the UCSCs in vitro. This indicates that UCSCs can effectively differentiate into the HLCs. In CCl4-intoxicated group, BUN, AST and ALT levels, and histological criteria, such as infiltration of inflammatory cells, accumulation of reticulocytes, nuclear pyknosis of hepatocyte and sinusoidal dilation, significantly increased. In this group, ALB secretion significantly decreased, while AFP expression significantly increased. Both UCSCs and HLCs encapsulated in alginate scaffolds effectively attenuated biochemical tests, improved liver cytoarchitecture, increased expression of ALB and reduced AFP expression. Conclusion: Finding of the present study indicated that encapsulation of UCSCs or HLCs in alginate mannuronic scaffolds effectively improve CCl4-induced ALF.

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    Cite

    APA: Copy

    Varaa, Negar, Azandeh, Saeed, KHORSANDI, LAYASADAT, BIJAN NEJAD, DARIOUSH, BAYATI, VAHID, & BAHREINI, AMIN. (2018). Ameliorating effect of encapsulated hepatocyte-like cells derived from umbilical cord in high mannuronic alginate scaffolds on acute liver failure in rats. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 21(9), 928-935. SID. https://sid.ir/paper/720747/en

    Vancouver: Copy

    Varaa Negar, Azandeh Saeed, KHORSANDI LAYASADAT, BIJAN NEJAD DARIOUSH, BAYATI VAHID, BAHREINI AMIN. Ameliorating effect of encapsulated hepatocyte-like cells derived from umbilical cord in high mannuronic alginate scaffolds on acute liver failure in rats. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2018;21(9):928-935. Available from: https://sid.ir/paper/720747/en

    IEEE: Copy

    Negar Varaa, Saeed Azandeh, LAYASADAT KHORSANDI, DARIOUSH BIJAN NEJAD, VAHID BAYATI, and AMIN BAHREINI, “Ameliorating effect of encapsulated hepatocyte-like cells derived from umbilical cord in high mannuronic alginate scaffolds on acute liver failure in rats,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 21, no. 9, pp. 928–935, 2018, [Online]. Available: https://sid.ir/paper/720747/en

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