Growth suppression effect of human mesenchymal stem cells from bone marrow, adipose tissue, and Wharton's jelly of umbilical cord on PBMCs

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AYATOLLAHI MARYAM; TALAEI KHOZANI TAHEREH; RAZMKHAH MAHBOOBEH

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Journal Paper

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Journal: Iranian Journal of Basic Medical Sciences Year:2016 | Volume:19 | Issue:2 Page(s): 145-153

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Title

Growth suppression effect of human mesenchymal stem cells from bone marrow, adipose tissue, and Wharton's jelly of umbilical cord on PBMCs

Author(s)

AYATOLLAHI MARYAM | TALAEI KHOZANI TAHEREH | RAZMKHAH MAHBOOBEH | Issue Writer Certificate

Keywords

Adipose tissue

Bone marrow

Immunosuppression

Mesenchymal stem cell (MSC)

Regenerative medicine

Wharton's jelly

Abstract

Objective(s): Immunosuppressive property of mesenchymal stem cells (MSCs) has great attraction in Regenerative medicine especially when dealing with tissue damage involving immune reactions. The most attractive tissue sources of MSCs used in clinical applications are Bone marrow (BM), Adipose tissue (AT), and Wharton's jelly (WJ) of human umbilical cord. The current study has compared immunomodulatory properties of human BM, AT, and WJ-MSCs. Materials and Methods: Three different types of human MSCs were isolated, cultured, and characterized by flow cytometry and differentiation potentials. The MSCs were co-cultured with allogeneic phytohemagglutinin (PHA) activated peripheral blood mononuclear cells (PBMCs). The proliferation of PBMCs was assessed by flow cytometry of carboxyfluorescein succinimidyl ester (CFSE) stained cells and compared to each other and to the growth of PBMCs in the absence of MSCs. Additionally, the growth suppression was indirectly assessed by using the transwell culture system. Results: The proliferation of PBMCs reduced to 6. 2, 7 and 15. 4-fold in cultures with AT-MSCs, WJ-MSCs, and BM-MSCs, respectively, compared to the PHA-activated cells. When the growth suppression was indirectly assessed by using the transwell culture system, it was revealed that AT-MSCs, WJ-MSCs, and BM-MSCs caused growth reduction in PBMCs to 3, 8, and 8-fold, respectively, compared to the PHA-activated cells. Conclusion: These data collectively conclude that the immunomodulatory effects of MSCs, which may mostly carry out through direct cell to cell contact, are different between various sources. Accordingly results of this study may contribute to the application of these cells in cell therapy and Regenerative medicine.

Cites

Fetal microchimerism in mouse caerulein-induced pancreatitis model

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APA: Copy

AYATOLLAHI, MARYAM, TALAEI KHOZANI, TAHEREH, & RAZMKHAH, MAHBOOBEH. (2016). Growth suppression effect of human mesenchymal stem cells from bone marrow, adipose tissue, and Wharton's jelly of umbilical cord on PBMCs. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 19(2), 145-153. SID. https://sid.ir/paper/720750/en

Vancouver: Copy

AYATOLLAHI MARYAM, TALAEI KHOZANI TAHEREH, RAZMKHAH MAHBOOBEH. Growth suppression effect of human mesenchymal stem cells from bone marrow, adipose tissue, and Wharton's jelly of umbilical cord on PBMCs. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2016;19(2):145-153. Available from: https://sid.ir/paper/720750/en

IEEE: Copy

MARYAM AYATOLLAHI, TAHEREH TALAEI KHOZANI, and MAHBOOBEH RAZMKHAH, “Growth suppression effect of human mesenchymal stem cells from bone marrow, adipose tissue, and Wharton's jelly of umbilical cord on PBMCs,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 19, no. 2, pp. 145–153, 2016, [Online]. Available: https://sid.ir/paper/720750/en

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