مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

228
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

135
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Up-regulation of TLR2 and TLR4 in high mobility group Box1-stimulated macrophages in pulpitis patients

Pages

  209-215

Keywords

Abstract

 High Mobility Group Box1 (HMGB1) is a nonhistone, DNA-binding protein that serves a crucial role in regulating gene transcription and is involved in a variety of proinflammatory, extracellular activities. The aim of this study was to explore whether HMGB1 stimulation can up-regulate the expression of Toll-like Receptor 2 (TLR2) and Toll-like Receptor 4 (TLR4) on macrophages from Pulpitis and to clarify the subsequent events involving Th17 cells and Th17 cell-associated cytokine changes. Materials and Methods: Having prepared dental pulp tissues of Pulpitis and healthy controls, macrophage were isolated and cultured. Macrophages were thereafter stimulated by HMGB1 time course. RT-QPCR, flowcytometer, immunofluorescence, Western blotting, and ELISA techniques were used in the present research. Results: Our results showed that the expression of TLR2 and TLR4 on macrophages stimulated with HMGB1 increased in Pulpitis compared with controls (macrophages without HMGB1 stimulation) with a statistical significance (P<0. 001). In addition, the levels of IL-17, IL-23, and IL-6 in supernatants from cultured macrophages stimulated with HMGB1 from Pulpitis increased, and NF-kB, the downstream target of TLR2 and TLR4, also showed a marked elevation after macrophages’ stimulation by HMGB1. Conclusion: The evidence from the present study suggests that the enhanced TLR2 and TLR4 pathways and Th17 cell polarization may be due to HMGB1 stimulation in Pulpitis.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    MAHMOUDI, JAVAD, SABERMAROUF, BABAK, BARADARAN, BEHZAD, Hatamnezhad, Leila Sadat, & SANDOGHCHIAN SHOTORBANI, SIAMAK. (2017). Up-regulation of TLR2 and TLR4 in high mobility group Box1-stimulated macrophages in pulpitis patients. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 20(2), 209-215. SID. https://sid.ir/paper/720871/en

    Vancouver: Copy

    MAHMOUDI JAVAD, SABERMAROUF BABAK, BARADARAN BEHZAD, Hatamnezhad Leila Sadat, SANDOGHCHIAN SHOTORBANI SIAMAK. Up-regulation of TLR2 and TLR4 in high mobility group Box1-stimulated macrophages in pulpitis patients. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2017;20(2):209-215. Available from: https://sid.ir/paper/720871/en

    IEEE: Copy

    JAVAD MAHMOUDI, BABAK SABERMAROUF, BEHZAD BARADARAN, Leila Sadat Hatamnezhad, and SIAMAK SANDOGHCHIAN SHOTORBANI, “Up-regulation of TLR2 and TLR4 in high mobility group Box1-stimulated macrophages in pulpitis patients,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 20, no. 2, pp. 209–215, 2017, [Online]. Available: https://sid.ir/paper/720871/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button