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Information Journal Paper

Title

Immunogenicity evaluation of plasmids encoding Brucella melitensis Omp25 and Omp31 antigens in BALB/c mice

Pages

  957-964

Abstract

 Objective(s): Vaccination is one of the most effective means to protect humans and animals against brucellosis. Live attenuated Brucella vaccines are considered effective in animals but they may be potentially infectious to humans, so it is vital to improve the immunoprotective effects and safety of vaccines against Brucella. This study was designed to evaluate the immunogenicity of DNA vaccines encoding B. melitensis outer membrane proteins (Omp25 and Omp31) against B. melitensis Rev1 in a mouse model. Materials and Methods: For this propose, Omp25 and Omp31 genes were cloned (individually and together) into the eukaryotic expression vector pcDNA3. 1/Hygro (+). Expressions of recombinant plasmids were confirmed by SDS-PAGE and Western blot analysis. Six groups of BALB/c mice (seven mice per group) were intramuscularly injected with three recombinant constructs, native pcDNA3. 1/ Hygro (+) and phosphate-buffered saline (PBS) as controls and subcutaneous injection of attenuated live vaccine Rev1. Results: Results indicated that DNA vaccine immunized BALB/c mice had a dominant immunoglobulin G response and elicited a T-cell-proliferative response and induced significant levels of interferon gamma (INF-γ ) compared to the control groups. Conclusion: Collectively, these finding suggested that the pcDNA3. 1/Hygro DNA vaccines encoding Omp25 and Omp31 genes and divalent plasmid were able to induce both humoral and cellular immunity, and had the potential to be a vaccine candidate for prevention of B. melitensis infections.

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    APA: Copy

    Shojaei, Moslem, TAHMOORESPUR, MOJTABA, Dehghani Soltani, Mahdi, & SEKHAVATI, MOHAMMAD HADI. (2018). Immunogenicity evaluation of plasmids encoding Brucella melitensis Omp25 and Omp31 antigens in BALB/c mice. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 21(9), 957-964. SID. https://sid.ir/paper/720908/en

    Vancouver: Copy

    Shojaei Moslem, TAHMOORESPUR MOJTABA, Dehghani Soltani Mahdi, SEKHAVATI MOHAMMAD HADI. Immunogenicity evaluation of plasmids encoding Brucella melitensis Omp25 and Omp31 antigens in BALB/c mice. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2018;21(9):957-964. Available from: https://sid.ir/paper/720908/en

    IEEE: Copy

    Moslem Shojaei, MOJTABA TAHMOORESPUR, Mahdi Dehghani Soltani, and MOHAMMAD HADI SEKHAVATI, “Immunogenicity evaluation of plasmids encoding Brucella melitensis Omp25 and Omp31 antigens in BALB/c mice,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 21, no. 9, pp. 957–964, 2018, [Online]. Available: https://sid.ir/paper/720908/en

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