Recombinant fibromodulin has therapeutic effects on diabetic nephropathy by down-regulating transforming growth factor-β 1 in streptozotocin-induced diabetic rat model

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Foroutan Jazi Maryam; BIGLARI ALIREZA; MAZLOOMZADEH SAEIDEH; Kingston Paul; RAMAZANI ALI; Tavkoli Bazzaz Javad; ESKANDARI MEHDI

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Journal: Iranian Journal of Basic Medical Sciences Year:2016 | Volume:19 | Issue:3 Page(s): 265-271

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Title

Recombinant fibromodulin has therapeutic effects on diabetic nephropathy by down-regulating transforming growth factor-β 1 in streptozotocin-induced diabetic rat model

Author(s)

Keywords

Diabetic rats

Diabetic nephropathy

Fibromodulin

Gene therapy

TGF-β1

Abstract

Objective(s): Diabetic nephropathy is an important long-term complication of diabetes mellitus which appears to be partially mediated by an increase in secretion of transforming growth factor-β (TGF-β ). Fibromodulin, the small leucine-rich proteoglycan, has been proposed to be the potent TGFβ 1 modulator. In this study, the therapeutic effects of recombinant adenoviral vectors expressing Fibromodulin on TGF-β 1 expression on Diabetic nephropathy were assessed. Materials and Methods: Forty-eight Sprague-Dawley rats were divided into 4 groups: STZ-induced Diabetic rats (diabetic-control), Fibromodulin adenovirus vector treated STZ rats (Ad-Fibromodulin), and Ad-lacZ-treated STZ rats (Ad-lacZ), and vehicle control (PBS-control). At 10 weeks after STZ treatment, we measured urinary albumin excretion (UAE), urine creatinine was measured by Jaffe method. We also measured kidney TGF-β 1 levels by reverse transcription polymerase chain reaction and Real-time PCR. Results: Urine albumin to creatinine ratio or UAE level were listed in four groups. UAE difference between healthy and Diabetic rats in all three groups were significant (P≤ 0. 005) and between the control group and treated groups were not significant. Our results indicated that TGF-β 1gene expression in Diabetic rats were increased and difference between normal group and diabetic group were significant (P≤ 0. 001). Fibromodulin gene transfection mediated by a recombinant adenovirus decreased TGF-β 1 level in STZ-induced Diabetic rats and TGF-β 1 mRNA in diabetic kidney were reduced 2 weeks after Ad-Fibromodulin injection. Conclusion: Intraperitoneal injection of adenoviral vectors expressing Fibromodulin reduced TGF-β 1 level in diabetic rat models. The molecular mechanisms involved in this process require further study.

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APA: Copy

Foroutan Jazi, Maryam, BIGLARI, ALIREZA, MAZLOOMZADEH, SAEIDEH, Kingston, Paul, RAMAZANI, ALI, Tavkoli Bazzaz, Javad, & ESKANDARI, MEHDI. (2016). Recombinant fibromodulin has therapeutic effects on diabetic nephropathy by down-regulating transforming growth factor-β 1 in streptozotocin-induced diabetic rat model. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 19(3), 265-271. SID. https://sid.ir/paper/721755/en

Vancouver: Copy

Foroutan Jazi Maryam, BIGLARI ALIREZA, MAZLOOMZADEH SAEIDEH, Kingston Paul, RAMAZANI ALI, Tavkoli Bazzaz Javad, ESKANDARI MEHDI. Recombinant fibromodulin has therapeutic effects on diabetic nephropathy by down-regulating transforming growth factor-β 1 in streptozotocin-induced diabetic rat model. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2016;19(3):265-271. Available from: https://sid.ir/paper/721755/en

IEEE: Copy

Maryam Foroutan Jazi, ALIREZA BIGLARI, SAEIDEH MAZLOOMZADEH, Paul Kingston, ALI RAMAZANI, Javad Tavkoli Bazzaz, and MEHDI ESKANDARI, “Recombinant fibromodulin has therapeutic effects on diabetic nephropathy by down-regulating transforming growth factor-β 1 in streptozotocin-induced diabetic rat model,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 19, no. 3, pp. 265–271, 2016, [Online]. Available: https://sid.ir/paper/721755/en

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