Relationship of cell surface hydrophobicity with biofilm formation and growth rate: A study on Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli

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Mirani Zulfiqar Ali; Fatima Aiman; Urooj Shaista; Aziz Mubashir; Naseem Khan Mohammad; Abbas Tanveer

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Journal Paper

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Journal: Iranian Journal of Basic Medical Sciences Year:2018 | Volume:21 | Issue:7 Page(s): 760-769

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Title

Relationship of cell surface hydrophobicity with biofilm formation and growth rate: A study on Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli

Author(s)

Keywords

Biofilms

Escherichia coli

Hydrophobicity

Pseudomonas aeruginosa

Small colony variants

Staphylococcus aureus

Abstract

Objective(s): This study was designed to determine the relationship of Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli isolates in multispecies Biofilms and their individual phenotypic characters in biofilm consortia. Materials and Methods: The subject isolates were recovered from different food samples and identified on the basis of growth on differential and selective media. Tube methods, Congo-red agar method, and scanning electron microscopy (SEM) were used to study Biofilms phenotypes. The Hydrophobicity of the strains was evaluated by the adhesion to apolar solvent. Results: The results showed that E. coli dominated the pre-biofilm stage. It has been observed that E. coli adopted biofilm life much before S. aureus and P. aeruginosa. However, after adopting biofilm lifestyle, slowly and gradually, P. aeruginosa dominated the consortia and dispersed other stakeholders. The subject isolates of P. aeruginosa produce cis-2-decanoic acid to disperse or inhibit S. aureus and E. coli Biofilms. Gas-chromatography and mass spectrometry results showed that cis-2-decanoic was higher in the co-culture condition and increased at late log-phase or at stationary phase. Although majority of S. aureus were unable to compete with P. aeruginosa, however, a minor population competed, survived, and persisted in biofilm consortia as Small colony variants. The survivors showed higher expression of sigB and sarA genes. P. aeruginosa showed comparatively higher hydrophobic surface properties. Conclusion: Comparative analysis showed that cell surface Hydrophobicity, growth rate, and Small colony variants (SCVs) are correlated in biofilm consortia of the P. aeruginosa, S. aureus, and E. coli.

Cites

Evaluation of Biofilm Formation and Frequency of Genes Encoding Curli Fiber, Colanic Acid Capsule and F1c Fimberia Among Uropathogenic Escherichia coli Isolates With Strong Cell Surface Hydrophobicity

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APA: Copy

Mirani, Zulfiqar Ali, Fatima, Aiman, Urooj, Shaista, Aziz, Mubashir, Naseem Khan, Mohammad, & Abbas, Tanveer. (2018). Relationship of cell surface hydrophobicity with biofilm formation and growth rate: A study on Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 21(7), 760-769. SID. https://sid.ir/paper/721810/en

Vancouver: Copy

Mirani Zulfiqar Ali, Fatima Aiman, Urooj Shaista, Aziz Mubashir, Naseem Khan Mohammad, Abbas Tanveer. Relationship of cell surface hydrophobicity with biofilm formation and growth rate: A study on Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2018;21(7):760-769. Available from: https://sid.ir/paper/721810/en

IEEE: Copy

Zulfiqar Ali Mirani, Aiman Fatima, Shaista Urooj, Mubashir Aziz, Mohammad Naseem Khan, and Tanveer Abbas, “Relationship of cell surface hydrophobicity with biofilm formation and growth rate: A study on Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 21, no. 7, pp. 760–769, 2018, [Online]. Available: https://sid.ir/paper/721810/en

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