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Information Journal Paper

Title

Nicotine-induced damages in testicular tissue of rats; evidences for bcl-2, p53 and caspase-3 expression

Pages

  199-208

Abstract

 Present study was performed in order to uncover new aspects for Nicotine-induced damages on spermatogenesis cell lineage. Materials and Methods: For this purpose, 36 mature male Wistar rats were divided into three groups as; control-sham (0. 2 ml, saline normal, IP), low dose (0. 2 mg/kg BW-1, IP) Nicotine-received and high dose (0. 4 mg/kg BW-1, IP) Nicotine-received groups. Following 7 weeks, the expression of Bcl-2, p53 and Caspase-3 at mRNA and protein levels were investigated by using reverse-transcriptase PCR (RT-PCR) and immunohistochemical (IHC) analyses, respectively. Moreover, the serum level of FSH, LH and Testosterone were evaluated. Finally, the mRNA damage was analyzed by using special fluorescent staining. Results: Nicotine, at both dose levels, decreased tubular differentiation, spermiogenesis and repopulation indices and enhanced cellular depletion. Animals in Nicotine-received groups exhibited a significant (P<0. 05) reduction at mRNA and protein levels of Bcl-2. More analyses revealed a remarkable (P<0. 05) enhancement in expression of p53 and Caspase-3 in comparison to control-sham animals. Finally, Nicotine resulted in a significant (P<0. 05) reduction in serum level of Testosterone and elevated mRNA damage. Conclusion: Our data showed that, Nicotine by suppressing the Testosterone biosynthesis, reducing mRNA and protein levels of Bcl-2 and up regulating the p53 and Caspase-3 mRNA and protein levels adversely affects the spermatogenesis and results in cellular depletion.

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    • Cite

    APA: Copy

    Mosadegh, Maryam, HASANZADEH, SHAPOUR, & RAZI, MAZDAK. (2017). Nicotine-induced damages in testicular tissue of rats; evidences for bcl-2, p53 and caspase-3 expression. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 20(2), 199-208. SID. https://sid.ir/paper/721889/en

    Vancouver: Copy

    Mosadegh Maryam, HASANZADEH SHAPOUR, RAZI MAZDAK. Nicotine-induced damages in testicular tissue of rats; evidences for bcl-2, p53 and caspase-3 expression. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2017;20(2):199-208. Available from: https://sid.ir/paper/721889/en

    IEEE: Copy

    Maryam Mosadegh, SHAPOUR HASANZADEH, and MAZDAK RAZI, “Nicotine-induced damages in testicular tissue of rats; evidences for bcl-2, p53 and caspase-3 expression,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 20, no. 2, pp. 199–208, 2017, [Online]. Available: https://sid.ir/paper/721889/en

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