Naringin enhances osteogenic differentiation through the activation of ERK signaling in human bone marrow mesenchymal stem cells

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Wang Huichao; Li Chunbo; Li Jianming; Zhu Yingjie; Jia Yudong; ZHANG YING; Zhang Xiaodong; LI WENLONG; Cui Lei; Li Wuyin; Liu Youwen

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Basic Medical Sciences Year:2017 | Volume:20 | Issue:4 Page(s): 407-413

Download Full-Text

View:

206

Download:

145

Cites:

Information Journal Paper

Title

Naringin enhances osteogenic differentiation through the activation of ERK signaling in human bone marrow mesenchymal stem cells

Author(s)

Keywords

Bone marrow-mesenchymal stem cells

ERK signaling pathway

Naringin

Osteogenic

differentiation

Abstract

Naringin has been reported to regulate bone metabolism. However, its effect on osteogenesis remains unclear. The aim was to investigate the effect of Naringin on Osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) through the activation of the ERK signaling pathway in Osteogenic differentiation. Materials and Methods: Annexin V-FITC assay and MTT assay were used to measure the effect of Naringin on cytotoxicity and proliferation of hBMSCs, respectively. Alkaline phosphatase activity analysis, Alizarin Red S staining, Western blotting, and real-time PCR assay were used to evaluate both the potential effect of Naringin on Osteogenic differentiation and the role of ERK signaling pathway in Osteogenic differentiation. Results: Our results showed that Naringin had no obvious toxicity on hBMSCs, and could significantly promote the proliferation of hBMSCs. Naringin also enhanced the Osteogenic differentiation of hBMSCs and increased the protein and mRNA expression levels of Osteogenic markers such as Runx-2, OXS, OCN, and Col1 in a dose-dependent manner. In addition, we found that the enhancing effect of Naringin on Osteogenic differentiation was related to the activation of phosphor-ERK, with an increase in duration of activity from 30 min to 120 min. More importantly, both the enhancing effect of Naringin on Osteogenic differentiation and the activity effect of Naringin on ERK signaling pathway were reversed by U0126 addition. Conclusion: Our findings demonstrated that Naringin promoted proliferation and osteogenesis of hBMSCs by activating the ERK signaling pathway and it might be a potential therapeutic agent for treating or preventing osteoporosis.

Cites

No record.

References

No record.

Cite

APA: Copy

Wang, Huichao, Li, Chunbo, Li, Jianming, Zhu, Yingjie, Jia, Yudong, ZHANG, YING, Zhang, Xiaodong, LI, WENLONG, Cui, Lei, Li, Wuyin, & Liu, Youwen. (2017). Naringin enhances osteogenic differentiation through the activation of ERK signaling in human bone marrow mesenchymal stem cells. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 20(4), 407-413. SID. https://sid.ir/paper/721892/en

Vancouver: Copy

Wang Huichao, Li Chunbo, Li Jianming, Zhu Yingjie, Jia Yudong, ZHANG YING, Zhang Xiaodong, LI WENLONG, Cui Lei, Li Wuyin, Liu Youwen. Naringin enhances osteogenic differentiation through the activation of ERK signaling in human bone marrow mesenchymal stem cells. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2017;20(4):407-413. Available from: https://sid.ir/paper/721892/en

IEEE: Copy

Huichao Wang, Chunbo Li, Jianming Li, Yingjie Zhu, Yudong Jia, YING ZHANG, Xiaodong Zhang, WENLONG LI, Lei Cui, Wuyin Li, and Youwen Liu, “Naringin enhances osteogenic differentiation through the activation of ERK signaling in human bone marrow mesenchymal stem cells,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 20, no. 4, pp. 407–413, 2017, [Online]. Available: https://sid.ir/paper/721892/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops