In vivo immunotherapy of lung cancer using cross-species reactive vascular endothelial growth factor nanobodies

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KAZEMI LOMEDASHT FATEMEH; POOSHANG BAGHERI KAMRAN; HABIBI ANBOUHI MAHDI; Hajizadeh Safar Ensiyeh; SHAHBAZZADEH DELAVAR; Mirzahosseini Hasan; BEHDANI MAHDI

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Journal: Iranian Journal of Basic Medical Sciences Year:2017 | Volume:20 | Issue:5 Page(s): 489-496

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Title

In vivo immunotherapy of lung cancer using cross-species reactive vascular endothelial growth factor nanobodies

Author(s)

Keywords

Angiogenesis Immunotherapy

Lung cancer

Nanobody

Vascular endothelial-growth factor

Abstract

Objective(s): Lung cancer is the main leading cause of cancer death worldwide. Angiogenesis is the main step in proliferation and spreading of tumor cells. Targeting vascular endothelial growth factor (VEGF) is an effective approach for inhibition of cancer angiogenesis. Nanobodies (NBs) are a novel class of antibodies derived from the camel. Unique characteristics of Nbs like their small size and good penetration to tumor tissues makes them promising tools in drug development. Development of NBs targeting both human and mouse VEGF is required for understanding their in vivo functions. Therefore, development of cross-species reactive anti-VEGF Nbs for immunotherapy of Lung cancer was the main aim of the current study. Materials and Methods: Here we developed NBs from Camelus dromedarius library with high specificity and binding affinity to both human and mouse VEGF. In vitro and In vivo function of developed NB was evaluated on human endothelial cells and lung epithelial tumor cells (TC-1). Results: A Nanobody showed the highest affinity to human and mouse VEGF and potently inhibited VEGF in the ELISA experiment. Anti-VEGF NBs significantly inhibited in vitro human endothelial cell migration through blockade of VEGF (P=0. 045). Anti-VEGF NBs also significantly inhibited in vivo TC-1 growth in a dose-dependent manner (P=0. 001) and resulted in higher survival rate in the Nanobody treated group Conclusion: These findings demonstrate the potential of anti-VEGF NBs in tumor growth inhibition and are promising as novel cancer therapeutic candidate.

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APA: Copy

KAZEMI LOMEDASHT, FATEMEH, POOSHANG BAGHERI, KAMRAN, HABIBI ANBOUHI, MAHDI, Hajizadeh Safar, Ensiyeh, SHAHBAZZADEH, DELAVAR, Mirzahosseini, Hasan, & BEHDANI, MAHDI. (2017). In vivo immunotherapy of lung cancer using cross-species reactive vascular endothelial growth factor nanobodies. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 20(5), 489-496. SID. https://sid.ir/paper/721922/en

Vancouver: Copy

KAZEMI LOMEDASHT FATEMEH, POOSHANG BAGHERI KAMRAN, HABIBI ANBOUHI MAHDI, Hajizadeh Safar Ensiyeh, SHAHBAZZADEH DELAVAR, Mirzahosseini Hasan, BEHDANI MAHDI. In vivo immunotherapy of lung cancer using cross-species reactive vascular endothelial growth factor nanobodies. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2017;20(5):489-496. Available from: https://sid.ir/paper/721922/en

IEEE: Copy

FATEMEH KAZEMI LOMEDASHT, KAMRAN POOSHANG BAGHERI, MAHDI HABIBI ANBOUHI, Ensiyeh Hajizadeh Safar, DELAVAR SHAHBAZZADEH, Hasan Mirzahosseini, and MAHDI BEHDANI, “In vivo immunotherapy of lung cancer using cross-species reactive vascular endothelial growth factor nanobodies,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 20, no. 5, pp. 489–496, 2017, [Online]. Available: https://sid.ir/paper/721922/en

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