Celecoxib, indomethacin, and ibuprofen prevent 6-hydroxydopamine-induced PC12 cell death through the inhibition of NFκ B and SAPK/JNK pathways

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Ramazani Elham; TAYARANI NAJARAN ZAHRA; FEREIDONI MASOUD

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Journal: Iranian Journal of Basic Medical Sciences Year:2019 | Volume:22 | Issue:5 Page(s): 478-484

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Title

Celecoxib, indomethacin, and ibuprofen prevent 6-hydroxydopamine-induced PC12 cell death through the inhibition of NFκ B and SAPK/JNK pathways

Author(s)

Ramazani Elham | TAYARANI NAJARAN ZAHRA | FEREIDONI MASOUD | Issue Writer Certificate

Keywords

Apoptosis

Glutathione

NSAIDs

Parkinson's disease

PC12 cells

ROS

Abstract

Objective(s): The possible action of nonsteroidal anti-inflammatory drugs (NSAIDs) in the reduction of reactive oxygen species (ROS) and also as anti-apoptotic agents may suggest them as putative agents for the treatment of neurodegenerative diseases. This study was designed to explore some pathways alterations induced by NSAIDs following 6-hydroxydopamine (6-OHDA)-induced cell death in PC12 cells as an in vitro model of Parkinson's disease (PD) and to compare the effects of celecoxib, indomethacin and ibuprofen. Materials and Methods: The cell viability, ROS content, Glutathione (GSH) level, and Apoptosis were measured using resazurin, dichlorofluorescein diacetate (DCFH-DA), 5, 5′-dithiobis-2-nitrobenzoic acid (DTNB), propidium iodide (PI) and flowcytometry, real-time PCR and western blot. Results: Based on the results, pretreatment with celecoxib, indomethacin and ibuprofen for 24 hr significantly induced concentration and time-dependent protection against 6-OHDA-induced PC12 cell death. Cell viability (P<0. 001), GSH level (P<0. 01) and cytoplasmic content of nuclear factor kappa B (NFκ B) (P<0. 01) were increased, also ROS content (P<0. 001) and Apoptosis biomarkers such as the cleaved caspase-3 (P<0. 001), Bax (P<0. 01), phospho-stress-activated protein kinases / c-Jun N-terminal kinases (P-SAPK/JNK) (P<0. 01) and cleaved poly ADP ribose polymerase (PARP) (P<0. 001) protein levels were all decreased after pretreatment of cells with NSAIDs in 6-OHDAinduced PC12 cells. Conclusion: It is suggested that NFκ B and SAPK/JNK pathways have an important role in 6-OHDAinduced cell injury. Overall, it seems that pretreatment with NSAIDs protect dopaminergic cells and may have the potential to slow the progression of PD.

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APA: Copy

Ramazani, Elham, TAYARANI NAJARAN, ZAHRA, & FEREIDONI, MASOUD. (2019). Celecoxib, indomethacin, and ibuprofen prevent 6-hydroxydopamine-induced PC12 cell death through the inhibition of NFκ B and SAPK/JNK pathways. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 22(5), 478-484. SID. https://sid.ir/paper/722343/en

Vancouver: Copy

Ramazani Elham, TAYARANI NAJARAN ZAHRA, FEREIDONI MASOUD. Celecoxib, indomethacin, and ibuprofen prevent 6-hydroxydopamine-induced PC12 cell death through the inhibition of NFκ B and SAPK/JNK pathways. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2019;22(5):478-484. Available from: https://sid.ir/paper/722343/en

IEEE: Copy

Elham Ramazani, ZAHRA TAYARANI NAJARAN, and MASOUD FEREIDONI, “Celecoxib, indomethacin, and ibuprofen prevent 6-hydroxydopamine-induced PC12 cell death through the inhibition of NFκ B and SAPK/JNK pathways,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 22, no. 5, pp. 478–484, 2019, [Online]. Available: https://sid.ir/paper/722343/en

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