Downregulation of microRNA-125a is involved in intervertebral disc degeneration by targeting pro-apoptotic Bcl-2 antagonist killer 1

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

LIU PING; Chang Feng; ZHANG TING; Gao Gang; Yu Chen; Ding Sheng Qiang; Zuo Gen Le; Huang Xin Hu

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Basic Medical Sciences Year:2017 | Volume:20 | Issue:11 Page(s): 1260-1267

Download Full-Text

View:

195

Download:

Cites:

Information Journal Paper

Title

Downregulation of microRNA-125a is involved in intervertebral disc degeneration by targeting pro-apoptotic Bcl-2 antagonist killer 1

Author(s)

Keywords

Apoptosis

BAK1 protein

Intervertebral disc-degeneration

Mir-125a

MicroRNA

Nucleus pulposus

Abstract

Objective(s): To investigate the role of the MicroRNA-125a (Mir-125a) and BAK1 in intervertebral disc degeneration (IDD). Materials and Methods: Degenerative lumbar Nucleus pulposus (NP) tissues were obtained from 193 patients who underwent resection, and normal controls consisted of normal NP tissues from 32 patients with traumatic lumbar fracture in our hospital. All patients were graded according to the Pfirrmann criteria. QRT-PCR was used to detect the expression of Mir-125a and BAK1, and Apoptosis of NP tissues detected by TUNEL staining. After isolation of non-degenerative and degenerative Nucleus pulposus cells (NPCs), the targeting relationship between Mir-125a and BAK1 was verified by dual luciferase reporter gene assay. Flow cytometry was determined the NPCs Apoptosis, and Western blot to measure the expressions of BAK1, Caspase-3, Bax and Bcl-2. Results: Mir-125a was reduced while BAK1 was elevated in IDD patients with the increase of Pfirrmann grade. Besides, miRNA-125a was negatively correlated to the NPCs Apoptosis, while BAK1 mRNA was positively correlated to cell Apoptosis. Additionally, BAK1 is the target gene of miRNA-125a. When transfection of Mir-125a mimics in vitro, the Apoptosis of NPCs were inhibited, with the down-regulation of BAK1, Caspase-3, and Bax, and the upregulation of Bcl-2. In addition, siBAK1 can reverse the pro-Apoptosis function of Mir-125a inhibitors in NPCs. Conclusion: miRNA-125a may regulate the Apoptosis status of the NPCs by inhibiting the expression of its target gene BAK1, which provided a potential strategy for further development of IDD therapies.

Cites

No record.

References

No record.

Cite

APA: Copy

LIU, PING, Chang, Feng, Gao, Gang, Yu, Chen, Ding, Sheng Qiang, Zuo, Gen Le, Huang, Xin Hu, & ZHANG, TING. (2017). Downregulation of microRNA-125a is involved in intervertebral disc degeneration by targeting pro-apoptotic Bcl-2 antagonist killer 1. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 20(11), 1260-1267. SID. https://sid.ir/paper/722664/en

Vancouver: Copy

LIU PING, Chang Feng, Gao Gang, Yu Chen, Ding Sheng Qiang, Zuo Gen Le, Huang Xin Hu, ZHANG TING. Downregulation of microRNA-125a is involved in intervertebral disc degeneration by targeting pro-apoptotic Bcl-2 antagonist killer 1. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2017;20(11):1260-1267. Available from: https://sid.ir/paper/722664/en

IEEE: Copy

PING LIU, Feng Chang, Gang Gao, Chen Yu, Sheng Qiang Ding, Gen Le Zuo, Xin Hu Huang, and TING ZHANG, “Downregulation of microRNA-125a is involved in intervertebral disc degeneration by targeting pro-apoptotic Bcl-2 antagonist killer 1,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 20, no. 11, pp. 1260–1267, 2017, [Online]. Available: https://sid.ir/paper/722664/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops