مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

239
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

110
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Attenuation by l-thyroxine of oxidant-induced gut epithelial damage

Pages

  1091-1096

Abstract

 Objective(s): Severe injuries are often associated with tissue hypothyroidism, elevated damaging mediators in circulation, and broken Gut epithelial barrier. However, the relationships between the hypothyroid state and Gut epithelial damage are largely unknown. Therefore, in this study, we investigated the effects of L-thyroxine (T4) on in vitro models of intact and compromised Gut epithelium. Materials and Methods: Gut epithelium equivalent was generated by cultivation of IEC-18 rat intestinal epithelial cells into transwell inserts. Confluent cultures were then compromised by scratching or H2O2 and Traumatized rat sera (TUR sera) treatments. Macromolecules permeation and transepithelial electrical resistance (TEER) were evaluated by conventional methods. Morphology and scratch wound closure were assessed microscopically. Cell viability/proliferation was assessed by MTT assay. Results: Both H2O2 and TUR sera induced marked yet different types of epithelial disintegration. While H2O2 significantly increased and decreased probe permeation and TEER, respectively, TUR sera was ineffective. Cultures treated with normal rat sera (sham sera) exhibited morphology, probe permeation, and TEER comparable to those of control cultures. Presence of T4 attenuated the H2O2-induced but not TUR sera-induced damages. T4 treatment accelerated, albeit marginally, wound closure but had virtually no effects on cell viability/proliferation. Conclusion: These data suggest that different mechanisms are involved in oxidant-and Traumainduced Gut epithelial barrier breakdown. Besides, they show that T4 markedly attenuates oxidantinduced Gut epithelial damage. Accordingly, one may also conclude that tissue hypothyroidism does not contribute to Trauma-induced Gut barrier breakdown.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    Shahedi, Zahra, Varedi, Masoumeh, Bagheri, Zohreh, Moatari, Afagh, & Sharafpour, Hengameh. (2019). Attenuation by l-thyroxine of oxidant-induced gut epithelial damage. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 22(9), 1091-1096. SID. https://sid.ir/paper/723422/en

    Vancouver: Copy

    Shahedi Zahra, Varedi Masoumeh, Bagheri Zohreh, Moatari Afagh, Sharafpour Hengameh. Attenuation by l-thyroxine of oxidant-induced gut epithelial damage. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2019;22(9):1091-1096. Available from: https://sid.ir/paper/723422/en

    IEEE: Copy

    Zahra Shahedi, Masoumeh Varedi, Zohreh Bagheri, Afagh Moatari, and Hengameh Sharafpour, “Attenuation by l-thyroxine of oxidant-induced gut epithelial damage,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 22, no. 9, pp. 1091–1096, 2019, [Online]. Available: https://sid.ir/paper/723422/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button