مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

294
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

322
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Platelets Apoptosis and Clearance in The Presence of Sodium Octanoate during Storage of Platelet Concentrate at 4˚ C

Pages

  212-217

Abstract

 Objective: Platelet (PLT) storage at 4˚ C has several benefits, however, it is accompanied by increased clearance of PLTs after transfusion. In this study, we evaluated the potential of sodium octanoate (SO) for reducing apoptosis and clearance rate of PLTs after long-term storage in cold. Materials and Methods: In this experimental study, PLT concentrates (PCs) were stored for 5 days under the following three conditions: 20-24˚ C, 4˚ C, and 4˚ C in the presence of SO. To measure the viability of PLTs, the water-soluble tetrazolium salt (WST-1) assay was performed. Phosphatidylserine (PS) exposure was determined on PLTs using flow cytometry technique. The amount of human active caspase-3 was determined in PLTs using an enzyme-linked immunosorbent assay. Additionally, the amount of PLT ingestion or clearance was determined by using HepG2 cell line. Results: The viability was higher in the SO-treated PLTs compared to the other groups. The level of PS exposure on PLTs was lower in the SO-treated PLTs compared to the other groups. The amount of active caspase-3 increased in all groups during 5-day storage. The highest increase in the amount of caspase-3 levels was observed at Cold Temperature. However, PLTs kept at 4˚ C in the presence of SO had a lower amount of active caspase-3 compared to PLTs kept at 4˚ C. The amount of PLTs removal by HepG2 cells was increased for 4˚ C-kept PLTs but it was lower for PLTs kept at 4˚ C in the presence of SO but, the differences were not significant (P>0. 05). Conclusion: SO could partially moderate the effects of Cold Temperature on apoptosis and viability of Platelets. It also decreases the ingestion rate of long-time refrigerated PLTs in vitro. Further studies using higher numbers of samples are required to demonstrate the effect of SO on reducing the clearance rate of PLTs.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    Baghdadi, Vahid, YARI, FATEMEH, NIKOUGOFTAR, MAHIN, & RAFIEE, MOHAMMAD HESSAM. (2020). Platelets Apoptosis and Clearance in The Presence of Sodium Octanoate during Storage of Platelet Concentrate at 4˚ C. CELL JOURNAL (YAKHTEH), 22(2), 212-217. SID. https://sid.ir/paper/725490/en

    Vancouver: Copy

    Baghdadi Vahid, YARI FATEMEH, NIKOUGOFTAR MAHIN, RAFIEE MOHAMMAD HESSAM. Platelets Apoptosis and Clearance in The Presence of Sodium Octanoate during Storage of Platelet Concentrate at 4˚ C. CELL JOURNAL (YAKHTEH)[Internet]. 2020;22(2):212-217. Available from: https://sid.ir/paper/725490/en

    IEEE: Copy

    Vahid Baghdadi, FATEMEH YARI, MAHIN NIKOUGOFTAR, and MOHAMMAD HESSAM RAFIEE, “Platelets Apoptosis and Clearance in The Presence of Sodium Octanoate during Storage of Platelet Concentrate at 4˚ C,” CELL JOURNAL (YAKHTEH), vol. 22, no. 2, pp. 212–217, 2020, [Online]. Available: https://sid.ir/paper/725490/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button